Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1779810..1780426 Replicon chromosome
Accession NZ_CP023466
Organism Pseudomonas frederiksbergensis strain KNU-15

Toxin (Protein)


Gene name hicA Uniprot ID A0A291AEE6
Locus tag CNN82_RS08470 Protein ID WP_019579596.1
Coordinates 1779810..1780022 (+) Length 71 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID J3A218
Locus tag CNN82_RS08475 Protein ID WP_008014960.1
Coordinates 1780022..1780426 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNN82_RS08445 1774836..1776839 + 2004 WP_019579592.1 heme lyase CcmF/NrfE family subunit -
CNN82_RS08450 1776836..1777372 + 537 WP_096479723.1 DsbE family thiol:disulfide interchange protein -
CNN82_RS08455 1777369..1777842 + 474 WP_018925074.1 cytochrome c-type biogenesis protein CcmH -
CNN82_RS08460 1777835..1779043 + 1209 WP_019579594.1 c-type cytochrome biogenesis protein CcmI -
CNN82_RS08465 1779058..1779456 + 399 WP_019579595.1 hypothetical protein -
CNN82_RS08470 1779810..1780022 + 213 WP_019579596.1 type II toxin-antitoxin system HicA family toxin Toxin
CNN82_RS08475 1780022..1780426 + 405 WP_008014960.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CNN82_RS08480 1780487..1781692 - 1206 WP_026331725.1 MFS transporter -
CNN82_RS08485 1781928..1782560 + 633 WP_019579598.1 hypothetical protein -
CNN82_RS08490 1782618..1783616 - 999 WP_019579599.1 sulfate ABC transporter substrate-binding protein -
CNN82_RS08495 1783723..1784547 - 825 WP_096479724.1 ion transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 71 a.a.        Molecular weight: 7834.11 Da        Isoelectric Point: 10.3517

>T81289 WP_019579596.1 NZ_CP023466:1779810-1780022 [Pseudomonas frederiksbergensis]
VQSRLLIKELMEAGWTLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPIGTVKSIRRRAGLYCPGASLAGDP

Download         Length: 213 bp

>T81289 NZ_CP023466:1779810-1780022 [Pseudomonas frederiksbergensis]
GTGCAGAGTAGGTTATTGATCAAGGAACTGATGGAGGCGGGCTGGACACTGGACCGGGTCACCGGCAGTCATCACATCTT
CACTCACCGTTACAACCCTTACACGATACCCGTCCCTCATCCGAAGAAGGATTTACCGATCGGGACGGTCAAAAGCATCA
GGAGGCGAGCCGGGCTTTATTGCCCGGGAGCCAGTTTGGCAGGAGATCCATAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14877.88 Da        Isoelectric Point: 4.3313

>AT81289 WP_008014960.1 NZ_CP023466:1780022-1780426 [Pseudomonas frederiksbergensis]
MQYPICIEWGDENTAIGIQIPDIPGAVTAGDTFEDAYNAAVEIAHIMLQEIMADGESIPMPTSAAAHRENPEFADMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRH

Download         Length: 405 bp

>AT81289 NZ_CP023466:1780022-1780426 [Pseudomonas frederiksbergensis]
ATGCAATACCCAATCTGTATCGAGTGGGGCGACGAGAACACCGCCATCGGTATTCAGATCCCCGATATTCCTGGTGCCGT
AACGGCCGGGGATACGTTTGAAGATGCTTACAACGCGGCAGTCGAGATTGCCCACATCATGCTGCAGGAGATCATGGCGG
ACGGGGAGTCAATTCCGATGCCGACCTCTGCGGCCGCGCATCGCGAGAATCCGGAATTTGCCGACATGGGTTGGGGGATG
CTGGAGCTGGACATCTCGCCGTATCTGGGCAAGACCGAGAAGGTCAACGTAACGCTGCCTGGCTACGTGATTCAGCGCAT
TGATCGTTATGTGCGAGAGCACAACGTCAAAAGTCGCTCCTCCTTTCTTGCGGATGCGGCGATGGAGAAGCTGGTTCGGC
ATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A291AEE6

Antitoxin

Source ID Structure
AlphaFold DB A0A291ADZ7

References