Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1493864..1494487 Replicon chromosome
Accession NZ_CP023466
Organism Pseudomonas frederiksbergensis strain KNU-15

Toxin (Protein)


Gene name hicA Uniprot ID A0A291ADC1
Locus tag CNN82_RS07135 Protein ID WP_019580871.1
Coordinates 1494308..1494487 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID J3BXS4
Locus tag CNN82_RS07130 Protein ID WP_008036180.1
Coordinates 1493864..1494271 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNN82_RS07105 1490487..1490945 + 459 WP_028939283.1 Lrp/AsnC family transcriptional regulator -
CNN82_RS07110 1491030..1491587 - 558 WP_157755493.1 hypothetical protein -
CNN82_RS07115 1491696..1492199 - 504 WP_019580873.1 inclusion body family protein -
CNN82_RS07120 1492642..1492971 + 330 WP_018926816.1 YXWGXW repeat-containing protein -
CNN82_RS07125 1493043..1493813 + 771 WP_028939285.1 trans-aconitate 2-methyltransferase -
CNN82_RS07130 1493864..1494271 - 408 WP_008036180.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CNN82_RS07135 1494308..1494487 - 180 WP_019580871.1 type II toxin-antitoxin system HicA family toxin Toxin
CNN82_RS07140 1494753..1495088 - 336 WP_018926814.1 hypothetical protein -
CNN82_RS07145 1495397..1496869 + 1473 WP_096479650.1 cytochrome c oxidase subunit II -
CNN82_RS07150 1496918..1498693 + 1776 WP_096479651.1 cytochrome c oxidase subunit I -
CNN82_RS07155 1498690..1499376 + 687 WP_096479652.1 cytochrome c oxidase subunit 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6681.93 Da        Isoelectric Point: 11.4113

>T81288 WP_019580871.1 NZ_CP023466:c1494487-1494308 [Pseudomonas frederiksbergensis]
VNSRFLISQIVADGWYLVRIRGSHHHFKHPTKPGLVTVPNPKKDLLKKTAISILQQALL

Download         Length: 180 bp

>T81288 NZ_CP023466:c1494487-1494308 [Pseudomonas frederiksbergensis]
GTGAATAGCCGGTTTTTGATAAGCCAAATCGTTGCGGACGGCTGGTATCTGGTACGAATCAGGGGGAGTCATCACCACTT
CAAGCATCCGACCAAGCCTGGACTGGTGACGGTTCCTAACCCGAAAAAGGACCTGCTCAAGAAAACGGCCATTAGTATTT
TGCAACAGGCGCTGCTCTGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14662.64 Da        Isoelectric Point: 4.3833

>AT81288 WP_008036180.1 NZ_CP023466:c1494271-1493864 [Pseudomonas frederiksbergensis]
MLYPIAISMGDDEHAWGVEVPDIPGCFSAGEDLDDAMAMAREAIEGHFEILAEDGSPIPPANKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQS

Download         Length: 408 bp

>AT81288 NZ_CP023466:c1494271-1493864 [Pseudomonas frederiksbergensis]
ATGCTTTACCCCATCGCGATTTCCATGGGTGATGACGAACACGCCTGGGGCGTAGAAGTTCCGGATATTCCAGGTTGCTT
CTCCGCCGGGGAAGATCTGGATGACGCCATGGCCATGGCTCGCGAAGCCATCGAAGGTCATTTCGAGATTCTGGCCGAAG
ACGGATCACCGATTCCGCCCGCCAACAAAGTGACCCTGCATGCGGCTAATCCACAGTACGCAGGGTGTACGTGGGCCGTG
GTGGATATCGATGTCACCAAATACCTGGGCAAGGCGCAGAAACTCAACATCACCCTGCCCGGCTATCTGCTCAACCGCAT
CGATGAGTACGTATTGCACCATCCGGAAGAGAAAAGTCGCTCCGGGTTTCTAGCCTCGGCGGCGCTCAAGGTCTTGCAGC
AAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A291ADC1

Antitoxin

Source ID Structure
AlphaFold DB A0A291ADB7

References