Detailed information of TA system TA081284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 90647..91178
• Replicon: plasmid p2
• Accession: NZ_CP023451
• Organism: Rhizorhabdus dicambivorans strain Ndbn-20

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T0GNF5
• Locus tag: CMV14_RS24930
• Protein ID: WP_004212690.1
• Coordinates: 90897..91178 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: T0G968
• Locus tag: CMV14_RS24925
• Protein ID: WP_004212689.1
• Coordinates: 90647..90910 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CMV14_RS24905	85822..87528	+	1707	WP_062343367.1	copper resistance system multicopper oxidase	-
CMV14_RS24910	87525..88511	+	987	WP_009823975.1	copper resistance protein B	-
CMV14_RS24915	88505..89746	+	1242	WP_066969879.1	MFS transporter	-
CMV14_RS24920	89826..90263	+	438	WP_007683347.1	metal-binding protein	-
CMV14_RS24925	90647..90910	+	264	WP_004212689.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CMV14_RS24930	90897..91178	+	282	WP_004212690.1	type II toxin-antitoxin system YafQ family toxin	Toxin
CMV14_RS26950	91499..91582	+	84	Protein_81	magnesium transporter	-
CMV14_RS24935	91580..92274	+	695	Protein_82	IS21 family transposase	-
CMV14_RS24940	92261..92989	+	729	WP_025549137.1	IS21-like element helper ATPase IstB	-
CMV14_RS24945	93161..93619	+	459	WP_004212694.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..256400	256400
	-	flank	IS/Tn	-	-	92261..92989	728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10993.49 Da
• Isoelectric Point: 7.4752

>T81284 WP_004212690.1 NZ_CP023451:90897-91178 [Rhizorhabdus dicambivorans]
MRTIERFGRFKRDYKREKKGQHAKTLDADLIPIIEALASDEPLEPRHRDHALTGDWRDHRDCHIKPDLVLIYRKPDDDTL
QLVRLGSHAELGW

Nucleotide

• Length: 282 bp

>T81284 NZ_CP023451:90897-91178 [Rhizorhabdus dicambivorans]
ATGCGGACGATTGAACGCTTCGGGCGGTTCAAGCGCGACTACAAGCGGGAGAAGAAGGGGCAGCACGCCAAGACGCTGGA
CGCTGACCTGATCCCGATCATCGAGGCGCTGGCATCCGACGAGCCGCTTGAACCGCGCCACCGCGACCATGCGCTGACCG
GCGACTGGCGCGACCATCGCGATTGCCACATAAAGCCCGACCTCGTGCTGATTTACCGCAAGCCCGATGACGACACCTTG
CAGCTTGTGCGCTTGGGATCGCACGCGGAATTGGGTTGGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9284.75 Da
• Isoelectric Point: 4.5881

>AT81284 WP_004212689.1 NZ_CP023451:90647-90910 [Rhizorhabdus dicambivorans]
MAANALVQTRIDGAVKEEAATVLAAMGLTVSDAVRLMLTRVARDKALPFEPLVPNAETIEAMKEAREGKGKRFATVVDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT81284 NZ_CP023451:90647-90910 [Rhizorhabdus dicambivorans]
ATGGCCGCAAACGCTCTTGTGCAGACCCGGATCGACGGAGCGGTGAAGGAGGAAGCCGCGACCGTGCTGGCTGCTATGGG
GCTGACCGTCTCCGATGCCGTGCGCCTGATGCTGACCCGCGTTGCCCGCGACAAGGCCTTGCCGTTTGAACCGCTGGTGC
CCAATGCCGAGACGATCGAGGCCATGAAGGAAGCGCGCGAGGGAAAGGGCAAGCGGTTCGCCACGGTGGTCGACCTGATG
GCCGACCTCAATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: T0GNF5

Antitoxin

• AlphaFold DB: T0G968