Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 3832919..3834567 Replicon chromosome
Accession NZ_CP023449
Organism Rhizorhabdus dicambivorans strain Ndbn-20

Toxin (Protein)


Gene name HipA3 Uniprot ID A0A2A4FU92
Locus tag CMV14_RS18020 Protein ID WP_066962658.1
Coordinates 3833266..3834567 (+) Length 434 a.a.

Antitoxin (Protein)


Gene name HipB3 Uniprot ID A0A2A4FWC8
Locus tag CMV14_RS18015 Protein ID WP_066962661.1
Coordinates 3832919..3833269 (+) Length 117 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CMV14_RS18000 3829833..3831062 - 1230 WP_066962664.1 three-Cys-motif partner protein TcmP -
CMV14_RS18005 3831082..3831864 - 783 WP_066962722.1 DUF5131 family protein -
CMV14_RS18010 3832370..3832762 + 393 WP_176489030.1 dual specificity protein phosphatase family protein -
CMV14_RS18015 3832919..3833269 + 351 WP_066962661.1 helix-turn-helix domain-containing protein Antitoxin
CMV14_RS18020 3833266..3834567 + 1302 WP_066962658.1 type II toxin-antitoxin system HipA family toxin Toxin
CMV14_RS18025 3834708..3834848 - 141 Protein_3586 PAS domain-containing protein -
CMV14_RS18030 3835360..3837129 + 1770 WP_066962719.1 single-stranded-DNA-specific exonuclease RecJ -
CMV14_RS18040 3837510..3838646 - 1137 WP_021246312.1 DUF3596 domain-containing protein -
CMV14_RS18045 3838607..3838810 - 204 WP_021246311.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3793286..3835028 41742


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(169-406)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-69)


Sequences

Toxin        


Download         Length: 434 a.a.        Molecular weight: 47372.34 Da        Isoelectric Point: 5.6566

>T81283 WP_066962658.1 NZ_CP023449:3833266-3834567 [Rhizorhabdus dicambivorans]
VTTAEVWLWGSRIGAVTLPTGERFASFEYDPAFQRSGIGLSPLNMPLGGVVYRFPALPTQSFHGLPGMLADALPDKYGNA
LIDAWLATQGRTSTDFNAVERLCYTGTRGMGALEFRPVQGPTSTKADSVEIEDLVKLASEVLVHRKDLRVTFASDEKAEA
LREILRVGTSAGGARAKAIIAWNADTNEVRSGQVQAPEGFGYWLMKFDGVANNKDHELADPQGFGAIEFAYSRMAAAAGI
EMAECRLFDEGGRRHFMTRRFDRQANGDKLHMQSLAALAHLDFNEPRANTYEQAFTTIRALGLGQDALDQQFRRAVFNIV
GRNQDDHVKNIAFLMDPAGNWRLAPAFDIAYAYNPGGDWTSQHQMSLAGQRDNFTLDSLIAGGKSAGVSARAVRTVLGEV
TEAIAQWRVIATDVGVPPGFVDDVEAHFRLDLR

Download         Length: 1302 bp

>T81283 NZ_CP023449:3833266-3834567 [Rhizorhabdus dicambivorans]
GTGACGACCGCTGAAGTCTGGTTGTGGGGCAGCCGCATCGGGGCGGTGACGCTGCCCACCGGCGAACGGTTCGCAAGCTT
TGAATATGATCCAGCATTCCAACGAAGCGGGATCGGTCTGTCTCCGTTGAACATGCCGCTAGGCGGTGTTGTATATCGGT
TTCCAGCGTTGCCCACGCAAAGTTTCCACGGCTTGCCCGGTATGCTCGCCGACGCCTTACCCGACAAATATGGCAATGCC
CTGATCGACGCATGGTTGGCGACCCAAGGCCGCACAAGTACGGATTTCAACGCGGTCGAGCGCCTTTGCTATACTGGCAC
ACGCGGTATGGGGGCGCTCGAGTTCCGGCCTGTTCAAGGACCTACATCAACCAAGGCAGATTCCGTCGAGATCGAGGACC
TAGTCAAGCTCGCTTCCGAAGTGCTCGTCCATCGCAAGGATCTGCGTGTCACGTTCGCGAGCGATGAAAAGGCGGAGGCG
CTGCGCGAAATCCTGCGCGTCGGTACATCCGCTGGCGGGGCGCGCGCGAAGGCGATTATCGCCTGGAACGCCGACACCAA
CGAAGTGCGTTCCGGGCAGGTACAGGCGCCGGAAGGCTTTGGCTACTGGCTGATGAAGTTCGACGGCGTTGCGAATAACA
AGGATCACGAACTTGCTGATCCCCAAGGATTTGGGGCGATCGAATTCGCCTATTCGAGGATGGCGGCGGCGGCCGGCATT
GAGATGGCAGAATGCCGGCTGTTCGATGAAGGCGGACGGCGGCATTTCATGACCCGCCGCTTCGACCGGCAGGCCAATGG
GGATAAGCTGCACATGCAGTCGCTCGCGGCCCTCGCCCATCTCGATTTCAATGAGCCGCGTGCCAATACATATGAGCAGG
CATTCACAACGATCCGAGCACTGGGGCTCGGTCAGGACGCGCTCGACCAGCAGTTTCGTCGGGCCGTATTCAACATCGTC
GGGCGTAACCAGGACGACCATGTTAAGAACATCGCCTTCTTGATGGATCCCGCTGGCAACTGGCGGCTCGCTCCGGCGTT
CGACATTGCCTACGCCTACAATCCAGGCGGCGATTGGACTTCTCAGCATCAAATGTCACTGGCGGGCCAGCGCGATAATT
TCACACTCGACAGCCTGATCGCCGGTGGCAAATCCGCTGGTGTATCAGCGCGAGCCGTCAGGACGGTCTTGGGTGAGGTA
ACGGAGGCGATTGCGCAGTGGCGTGTCATCGCCACGGATGTCGGAGTGCCGCCCGGCTTTGTCGATGACGTCGAAGCCCA
CTTCCGGCTCGATCTTAGATGA

Antitoxin


Download         Length: 117 a.a.        Molecular weight: 12992.95 Da        Isoelectric Point: 11.1119

>AT81283 WP_066962661.1 NZ_CP023449:3832919-3833269 [Rhizorhabdus dicambivorans]
MQIMSAMPEATKLAELGQRAQQYRVGMNLTQVELARHAGVSRSTVERFEAGSSVQLDKLLRILGALRLSDNLNQLIPEAG
IRPIQLADSKVETRQRAYKRRSASAKEQGWTWGKKT

Download         Length: 351 bp

>AT81283 NZ_CP023449:3832919-3833269 [Rhizorhabdus dicambivorans]
ATGCAAATCATGTCCGCAATGCCTGAGGCGACAAAGCTGGCCGAACTCGGCCAGCGTGCGCAGCAATATCGCGTTGGAAT
GAATCTCACTCAGGTCGAACTGGCCAGGCATGCTGGGGTGTCGCGCAGCACGGTCGAACGATTCGAAGCAGGAAGTTCGG
TTCAGCTCGACAAACTGCTGCGGATATTGGGAGCGCTCAGGCTGTCCGACAATCTCAACCAGTTGATTCCAGAGGCAGGC
ATTCGTCCGATTCAACTGGCAGATTCCAAGGTCGAGACCCGGCAGCGCGCCTACAAGCGCAGGAGCGCTTCGGCGAAGGA
GCAAGGCTGGACCTGGGGCAAGAAGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4FU92

Antitoxin

Source ID Structure
AlphaFold DB A0A2A4FWC8

References