Detailed information of TA system TA081281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2709568..2710241
• Replicon: chromosome
• Accession: NZ_CP023449
• Organism: Rhizorhabdus dicambivorans strain Ndbn-20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CMV14_RS12810
• Protein ID: WP_066970156.1
• Coordinates: 2709819..2710241 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0G3XGK1
• Locus tag: CMV14_RS12805
• Protein ID: WP_012110103.1
• Coordinates: 2709568..2709822 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CMV14_RS12795	2707173..2708207	+	1035	WP_066970150.1	toprim domain-containing protein	-
CMV14_RS12800	2708555..2709481	+	927	WP_066970153.1	DUF2493 domain-containing protein	-
CMV14_RS12805	2709568..2709822	+	255	WP_012110103.1	plasmid stabilization protein	Antitoxin
CMV14_RS12810	2709819..2710241	+	423	WP_066970156.1	type II toxin-antitoxin system VapC family toxin	Toxin
CMV14_RS26765	2710523..2710690	+	168	WP_168168942.1	hypothetical protein	-
CMV14_RS12815	2710825..2711265	+	441	WP_066970161.1	hypothetical protein	-
CMV14_RS12825	2711834..2712160	+	327	WP_041535876.1	DUF736 domain-containing protein	-
CMV14_RS12830	2712559..2713137	+	579	WP_202820847.1	TnsA endonuclease N-terminal domain-containing protein	-
CMV14_RS12835	2713134..2714816	+	1683	WP_066970322.1	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14936.07 Da
• Isoelectric Point: 4.5082

>T81281 WP_066970156.1 NZ_CP023449:2709819-2710241 [Rhizorhabdus dicambivorans]
MILLDTNVVSEAMKPAPDEAVRAWLDEQAAETLFLSSVTIAELMFGIGALPEGKRKERLTEALDGVMELFADRVLPFDVD
AARRYADIAVKARAAGKGFPTPDGYIAAIAAAKGFAVATRDNSAFDAANVTVIDPWKAGH

Nucleotide

• Length: 423 bp

>T81281 NZ_CP023449:2709819-2710241 [Rhizorhabdus dicambivorans]
ATGATCCTCCTCGACACCAATGTCGTATCGGAGGCGATGAAGCCCGCTCCCGACGAGGCCGTGCGCGCATGGCTCGACGA
GCAGGCGGCGGAAACACTCTTTCTCTCCAGCGTCACCATCGCCGAGCTGATGTTCGGCATCGGCGCGCTCCCCGAGGGCA
AGCGCAAGGAGCGTCTCACCGAAGCCCTAGATGGGGTGATGGAACTGTTCGCAGACCGCGTCCTCCCGTTCGATGTCGAT
GCTGCGCGCCGCTATGCGGATATCGCCGTCAAAGCCCGAGCGGCCGGGAAAGGCTTCCCTACTCCGGACGGCTACATCGC
GGCGATCGCAGCCGCCAAGGGTTTTGCCGTCGCCACGCGCGACAACAGCGCATTCGACGCCGCGAATGTCACGGTCATCG
ATCCCTGGAAGGCCGGGCACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9351.67 Da
• Isoelectric Point: 10.4317

>AT81281 WP_012110103.1 NZ_CP023449:2709568-2709822 [Rhizorhabdus dicambivorans]
MPAVTIRNLSDATHRALKVRAAQHGRSAEAEMRDILEMAVRPDTRLRLGTALAERSRRLGLTNEDVEALDQARDKAPAKP
MSFE

Nucleotide

• Length: 255 bp

>AT81281 NZ_CP023449:2709568-2709822 [Rhizorhabdus dicambivorans]
ATGCCCGCAGTTACGATCAGGAATCTGTCCGACGCGACGCACCGCGCCCTCAAGGTGCGGGCGGCGCAGCACGGCCGCAG
CGCCGAAGCGGAAATGCGCGACATCCTCGAAATGGCTGTCCGCCCCGATACGCGCCTCCGGCTCGGCACGGCGCTCGCGG
AACGCAGCCGCCGCCTCGGCTTGACCAATGAGGATGTCGAGGCCCTCGACCAGGCTCGTGACAAGGCGCCCGCCAAGCCG
ATGAGCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0G3XGK1