Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 889380..889924 Replicon chromosome
Accession NZ_CP023449
Organism Rhizorhabdus dicambivorans strain Ndbn-20

Toxin (Protein)


Gene name relE Uniprot ID A0A2A4FZI2
Locus tag CMV14_RS04290 Protein ID WP_066959455.1
Coordinates 889380..889658 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2A4FZQ2
Locus tag CMV14_RS04295 Protein ID WP_066959727.1
Coordinates 889655..889924 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CMV14_RS04270 884765..886024 + 1260 WP_066959463.1 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) subunit alpha -
CMV14_RS04275 886161..887165 + 1005 WP_066959461.1 alpha-ketoacid dehydrogenase subunit beta -
CMV14_RS04280 887171..888466 + 1296 WP_066959459.1 2-oxo acid dehydrogenase subunit E2 -
CMV14_RS04285 888640..889383 + 744 WP_066959457.1 YebC/PmpR family DNA-binding transcriptional regulator -
CMV14_RS04290 889380..889658 - 279 WP_066959455.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CMV14_RS04295 889655..889924 - 270 WP_066959727.1 ribbon-helix-helix protein, CopG family Antitoxin
CMV14_RS04300 890046..890543 + 498 WP_066959453.1 crossover junction endodeoxyribonuclease RuvC -
CMV14_RS04305 890540..891139 + 600 WP_066959451.1 Holliday junction branch migration protein RuvA -
CMV14_RS04310 891294..892325 + 1032 WP_066959450.1 Holliday junction branch migration DNA helicase RuvB -
CMV14_RS04315 892510..892953 + 444 WP_066959449.1 tol-pal system-associated acyl-CoA thioesterase -
CMV14_RS04320 892973..893671 + 699 WP_066959447.1 protein TolQ -
CMV14_RS04325 893671..894111 + 441 WP_066959446.1 protein TolR -
CMV14_RS04330 894108..894920 + 813 WP_066959444.1 cell envelope integrity protein TolA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(8-44)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10802.55 Da        Isoelectric Point: 10.0829

>T81280 WP_066959455.1 NZ_CP023449:c889658-889380 [Rhizorhabdus dicambivorans]
MKIEWSNQAVTDLARLHAFLAPDAPEAAARMLQQMGRAPGRLIDFPRLGEKLDLYLPREVRRIKVGRYEMRYEIADTTIT
ILRLWHSREDRP

Download         Length: 279 bp

>T81280 NZ_CP023449:c889658-889380 [Rhizorhabdus dicambivorans]
ATGAAAATTGAGTGGTCGAACCAGGCGGTCACTGATCTCGCGCGGCTACATGCATTTCTGGCCCCTGACGCCCCCGAGGC
CGCCGCGCGAATGCTGCAACAAATGGGGCGAGCGCCGGGCAGGCTGATCGACTTTCCACGTCTTGGCGAAAAGCTCGACC
TCTATCTGCCTCGCGAGGTGCGGCGGATCAAAGTCGGTCGCTATGAAATGCGCTATGAGATAGCGGACACTACGATCACG
ATCCTGCGGCTGTGGCACAGCCGCGAAGATCGCCCTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9938.37 Da        Isoelectric Point: 4.8728

>AT81280 WP_066959727.1 NZ_CP023449:c889924-889655 [Rhizorhabdus dicambivorans]
MNEATKVLTAHVPVDLAEKVEAMASRLERSRGWVVKQALAAWVDLEEERHRMTLEGLADVDAGRVIDHETVLRWVDSLGT
SEPLPPPIK

Download         Length: 270 bp

>AT81280 NZ_CP023449:c889924-889655 [Rhizorhabdus dicambivorans]
ATGAACGAAGCAACCAAAGTGCTTACGGCACATGTGCCTGTCGATTTGGCAGAAAAAGTCGAGGCGATGGCCTCACGGCT
CGAGCGATCACGAGGCTGGGTGGTGAAGCAGGCCTTGGCAGCATGGGTCGATCTTGAGGAGGAACGCCATCGCATGACGC
TTGAAGGCTTGGCAGATGTCGATGCCGGCCGCGTGATCGATCATGAGACGGTGCTTCGTTGGGTCGATAGCCTCGGCACG
AGCGAGCCGCTGCCGCCGCCCATCAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4FZI2

Antitoxin

Source ID Structure
AlphaFold DB A0A2A4FZQ2

References