Detailed information of TA system TA081277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 71985..72637
• Replicon: plasmid pTX1
• Accession: NZ_CP023440
• Organism: Thauera sp. K11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCZ27_RS22835
• Protein ID: WP_096453040.1
• Coordinates: 72221..72637 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: CCZ27_RS22830
• Protein ID: WP_096453148.1
• Coordinates: 71985..72221 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCZ27_RS22795	67952..68620	-	669	WP_096453031.1	SOS response-associated peptidase	-
CCZ27_RS22800	68709..69041	+	333	WP_096453033.1	hypothetical protein	-
CCZ27_RS22805	69045..69266	-	222	Protein_70	hypothetical protein	-
CCZ27_RS22815	70094..70237	-	144	WP_198363388.1	hypothetical protein	-
CCZ27_RS22820	70452..70946	+	495	WP_096453037.1	H-NS histone family protein	-
CCZ27_RS22825	71171..71719	-	549	WP_096453038.1	TIGR02281 family clan AA aspartic protease	-
CCZ27_RS22830	71985..72221	+	237	WP_096453148.1	AbrB family transcriptional regulator	Antitoxin
CCZ27_RS22835	72221..72637	+	417	WP_096453040.1	type II toxin-antitoxin system VapC family toxin	Toxin
CCZ27_RS22840	73120..73737	+	618	WP_096453042.1	DNA-binding protein	-
CCZ27_RS22845	73968..74363	-	396	WP_096453044.1	type II toxin-antitoxin system VapC family toxin	-
CCZ27_RS22850	74360..74611	-	252	WP_096453046.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..140963	140963

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15123.38 Da
• Isoelectric Point: 4.7452

>T81277 WP_096453040.1 NZ_CP023440:72221-72637 [Thauera sp. K11]
MARYMLDTNMCIYLMKNQPEAVARRFAQCYVGDVVMSAITYAELEYGVAVCADPEREKANLAALIEDIPVVAFDAAAGVA
YGPIRLATRENKKDALDKLIAAHAVSIGVTVVTNNTKDFVRYPGVAVENWLEPEKDGH

Nucleotide

• Length: 417 bp

>T81277 NZ_CP023440:72221-72637 [Thauera sp. K11]
ATGGCGCGCTACATGCTCGACACGAACATGTGCATCTACCTGATGAAAAACCAGCCCGAGGCGGTCGCCCGGCGCTTCGC
ACAGTGCTACGTCGGTGACGTGGTCATGTCAGCGATCACCTACGCCGAACTGGAGTACGGCGTAGCTGTGTGTGCGGATC
CAGAGCGGGAAAAGGCCAATCTGGCAGCGCTTATTGAGGATATTCCCGTCGTTGCGTTCGATGCAGCGGCGGGAGTAGCG
TACGGACCGATCCGGCTTGCAACCCGCGAGAACAAGAAGGATGCGCTCGACAAGCTGATCGCCGCTCACGCGGTCTCCAT
CGGCGTGACGGTCGTGACCAACAACACCAAGGACTTTGTCAGGTATCCCGGCGTAGCGGTTGAGAACTGGCTGGAGCCCG
AAAAGGATGGTCACTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8949.95 Da
• Isoelectric Point: 5.1651

>AT81277 WP_096453148.1 NZ_CP023440:71985-72221 [Thauera sp. K11]
MHTTRAFKNGNSQAVRIPADLAYDRTDIELEIEREGDEIRIRPARRSLSGVLSKFAKFAPEFMEQGRGDQEQNERDAL

Nucleotide

• Length: 237 bp

>AT81277 NZ_CP023440:71985-72221 [Thauera sp. K11]
ATGCACACGACACGCGCGTTCAAGAACGGCAACTCACAAGCCGTCCGCATCCCCGCCGACCTGGCTTACGACAGAACGGA
CATCGAGCTTGAGATCGAACGGGAAGGTGACGAGATCCGCATCCGTCCGGCGCGTCGATCGCTTTCGGGGGTGCTCTCGA
AATTCGCCAAATTCGCCCCGGAGTTCATGGAACAAGGTCGTGGCGACCAGGAGCAAAATGAAAGGGACGCACTGTGA