Detailed information of TA system TA081276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RHH
• Location: 29518..30071
• Replicon: plasmid pTX1
• Accession: NZ_CP023440
• Organism: Thauera sp. K11

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CCZ27_RS22620
• Protein ID: WP_096452975.1
• Coordinates: 29778..30071 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CCZ27_RS22615
• Protein ID: WP_096452973.1
• Coordinates: 29518..29790 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCZ27_RS22590	24684..25121	+	438	WP_096452963.1	conjugal transfer protein TraM	-
CCZ27_RS22595	25569..25922	-	354	WP_096452965.1	conjugal transfer protein TraO	-
CCZ27_RS22600	26316..26885	-	570	WP_096452967.1	DNA repair protein RadC	-
CCZ27_RS22605	27681..28217	+	537	WP_096452969.1	phasin family protein	-
CCZ27_RS22610	28355..28645	-	291	WP_157748692.1	hypothetical protein	-
CCZ27_RS23825	29005..29433	+	429	WP_157748693.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
CCZ27_RS22615	29518..29790	+	273	WP_096452973.1	CopG family ribbon-helix-helix protein	Antitoxin
CCZ27_RS22620	29778..30071	+	294	WP_096452975.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCZ27_RS22625	30177..31784	+	1608	WP_096452977.1	type I restriction-modification system subunit M	-
CCZ27_RS23830	31781..32056	+	276	WP_157748694.1	hypothetical protein	-
CCZ27_RS22630	32185..32937	-	753	WP_096452979.1	IS21-like element helper ATPase IstB	-
CCZ27_RS22635	32934..34475	-	1542	WP_096452981.1	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..140963	140963

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10774.33 Da
• Isoelectric Point: 6.2244

>T81276 WP_096452975.1 NZ_CP023440:29778-30071 [Thauera sp. K11]
VPHVIVTAGAAEGLERCREFLATKAPEAARRAGQAIERQLRLLETAPDIGRPFPDMPELRELVIAFGDSGYLALYRHEPT
VDAVYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T81276 NZ_CP023440:29778-30071 [Thauera sp. K11]
GTGCCACACGTAATCGTTACCGCAGGCGCAGCGGAAGGCTTGGAGCGATGCCGCGAGTTCCTGGCCACCAAAGCACCTGA
AGCCGCTCGGCGGGCGGGTCAGGCGATCGAGCGGCAATTGCGGCTGCTGGAAACAGCTCCCGACATTGGCAGACCGTTTC
CTGACATGCCTGAACTGCGCGAGTTAGTGATTGCCTTCGGGGACTCCGGCTATCTCGCACTGTATCGGCATGAACCGACC
GTTGATGCGGTGTATGTCTTGGCATTCCGGCATCAGAAAGAGGCCGGGTACTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10442.67 Da
• Isoelectric Point: 6.2220

>AT81276 WP_096452973.1 NZ_CP023440:29518-29790 [Thauera sp. K11]
MATSLKIDDALKGRVQQLAGQRRRSAHWIMLEAIQQYVEREEARERFKQEALASWVAYQETGQHLTGQEVRTWLNTWGTE
DETTVPACHT

Nucleotide

• Length: 273 bp

>AT81276 NZ_CP023440:29518-29790 [Thauera sp. K11]
ATGGCAACCTCGCTCAAGATTGACGACGCGTTGAAAGGTCGCGTCCAGCAACTCGCCGGTCAGCGCCGCCGCTCGGCACA
CTGGATCATGCTTGAAGCGATCCAGCAGTACGTCGAGCGTGAGGAAGCCCGTGAACGCTTCAAACAGGAAGCCTTGGCAT
CGTGGGTGGCCTATCAAGAAACAGGCCAGCACCTGACAGGGCAGGAAGTTCGCACCTGGTTGAACACCTGGGGCACCGAA
GACGAGACGACGGTGCCTGCGTGCCACACGTAA