Detailed information of TA system TA081264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 4951304..4951960
• Replicon: chromosome
• Accession: NZ_CP023435
• Organism: Mycobacterium sp. PYR15

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: CAC50_RS24190
• Protein ID: WP_096312225.1
• Coordinates: 4951304..4951750 (-)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A1J0UF69
• Locus tag: CAC50_RS24195
• Protein ID: WP_071949325.1
• Coordinates: 4951754..4951960 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAC50_RS24165	4948011..4948895	+	885	WP_096312222.1	hypothetical protein	-
CAC50_RS24170	4948892..4949779	-	888	WP_096312223.1	amidohydrolase family protein	-
CAC50_RS24175	4949888..4950547	+	660	WP_071949331.1	response regulator transcription factor	-
CAC50_RS24180	4950549..4950776	-	228	WP_071949330.1	hypothetical protein	-
CAC50_RS24185	4950917..4951300	+	384	WP_096312224.1	hypothetical protein	-
CAC50_RS24190	4951304..4951750	-	447	WP_096312225.1	SRPBCC family protein	Toxin
CAC50_RS24195	4951754..4951960	-	207	WP_071949325.1	antitoxin	Antitoxin
CAC50_RS24200	4951964..4954363	-	2400	WP_096312226.1	HAD-IC family P-type ATPase	-
CAC50_RS24205	4954360..4955946	-	1587	WP_096312227.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-145)

Antitoxin

• Antitoxin Rv0909/MT0933 (9-55)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 16052.73 Da
• Isoelectric Point: 10.1634

>T81264 WP_096312225.1 NZ_CP023435:c4951750-4951304 [Mycobacterium sp. PYR15]
MAKLSVSVDVPLPPEQAWQHASDLSRYKDWLTIHRVWRSKLPDTLEKGTVVESIVEVKGMPNRVKWEIVHFKAPEAMTLN
GAGVGGVKVKLIGKVRANDKDPNTSVVTMDVHLGGAALFGPIGMVVAGALKGDIKESLNRFVTVFAPS

Nucleotide

• Length: 447 bp

>T81264 NZ_CP023435:c4951750-4951304 [Mycobacterium sp. PYR15]
ATGGCGAAACTCTCAGTCTCCGTTGACGTTCCACTTCCACCAGAGCAGGCCTGGCAGCACGCCTCGGACCTCTCCCGCTA
CAAGGACTGGCTGACCATCCACCGGGTGTGGCGCTCCAAACTGCCCGACACCCTCGAGAAGGGCACCGTCGTGGAGTCCA
TCGTCGAGGTCAAAGGCATGCCCAACCGGGTGAAGTGGGAGATCGTCCACTTCAAGGCCCCCGAGGCCATGACGCTCAAC
GGCGCCGGCGTCGGCGGGGTGAAGGTCAAGCTGATCGGCAAGGTGCGGGCCAACGACAAGGACCCCAACACTTCGGTGGT
GACCATGGACGTCCATCTGGGCGGTGCGGCGCTGTTCGGGCCGATCGGCATGGTCGTGGCCGGTGCGCTCAAGGGCGACA
TCAAGGAGTCGCTGAACCGCTTTGTGACGGTATTCGCGCCGTCGTAA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7374.13 Da
• Isoelectric Point: 5.0152

>AT81264 WP_071949325.1 NZ_CP023435:c4951960-4951754 [Mycobacterium sp. PYR15]
MGFLDKALGKTKEVLGSNADKVEQAIDKAGDIIDQKTQGKYSSTVDKVQDAAKNYVDSNRTDKGEQPN

Nucleotide

• Length: 207 bp

>AT81264 NZ_CP023435:c4951960-4951754 [Mycobacterium sp. PYR15]
ATGGGATTCCTCGACAAAGCCCTCGGCAAGACCAAGGAAGTTCTGGGCTCCAATGCCGACAAGGTGGAGCAGGCCATCGA
CAAGGCCGGCGACATCATCGATCAGAAGACGCAGGGCAAGTACTCCAGCACCGTCGACAAGGTGCAGGACGCCGCCAAGA
ACTACGTCGACTCCAACCGCACCGACAAAGGCGAGCAACCCAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A1J0UF69