Detailed information of TA system TA081260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 905110..905687
• Replicon: chromosome
• Accession: NZ_CP023435
• Organism: Mycobacterium sp. PYR15

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A1J0UAG7
• Locus tag: CAC50_RS04255
• Protein ID: WP_071947609.1
• Coordinates: 905304..905687 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: CAC50_RS04250
• Protein ID: WP_096312685.1
• Coordinates: 905110..905307 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAC50_RS04220	900661..901695	-	1035	WP_096312684.1	hypothetical protein	-
CAC50_RS04225	901786..902430	+	645	WP_096309959.1	NUDIX hydrolase	-
CAC50_RS04230	902509..903759	+	1251	WP_096309961.1	cytochrome P450	-
CAC50_RS04235	903823..904248	+	426	WP_096309963.1	hypothetical protein	-
CAC50_RS04240	904333..904728	-	396	WP_096309965.1	type II toxin-antitoxin system VapC family toxin	-
CAC50_RS04245	904729..904977	-	249	WP_071947610.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
CAC50_RS04250	905110..905307	+	198	WP_096312685.1	antitoxin Phd	Antitoxin
CAC50_RS04255	905304..905687	+	384	WP_071947609.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CAC50_RS04260	905767..906345	+	579	WP_096309967.1	hypothetical protein	-
CAC50_RS04265	906404..907315	+	912	WP_096309969.1	hypothetical protein	-
CAC50_RS04270	907378..907641	+	264	WP_096309971.1	type II toxin-antitoxin system VapB family antitoxin	-
CAC50_RS04275	907638..908036	+	399	WP_096309973.1	type II toxin-antitoxin system VapC family toxin	-
CAC50_RS04280	908078..909736	+	1659	WP_125477387.1	hypothetical protein	-
CAC50_RS04285	909747..910229	-	483	WP_164520017.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	895551..909736	14185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13535.20 Da
• Isoelectric Point: 3.8694

>T81260 WP_071947609.1 NZ_CP023435:905304-905687 [Mycobacterium sp. PYR15]
VTEYLDRDDVLVAGAAAVGVELRVADYGLLDAAVARPQATVFGVDAYPDLVTKAAALLQSLARNHALVDGNKRTAWAAAW
TFLYINGVELNPDFDVDDAEDFMNAVAVQGDLELPELAEKLQGFAIG

Nucleotide

• Length: 384 bp

>T81260 NZ_CP023435:905304-905687 [Mycobacterium sp. PYR15]
GTGACCGAATACCTGGACCGTGACGATGTCCTGGTCGCCGGTGCTGCGGCGGTGGGGGTCGAGCTGAGAGTGGCCGACTA
CGGCCTGCTCGATGCGGCGGTGGCGCGCCCGCAGGCTACCGTCTTCGGTGTTGACGCCTATCCTGACCTCGTCACCAAGG
CGGCGGCGCTGCTGCAGTCTTTGGCTCGCAACCACGCACTGGTCGACGGCAACAAGCGCACCGCATGGGCAGCCGCGTGG
ACGTTCCTGTACATCAACGGAGTCGAGTTGAACCCCGATTTCGATGTCGACGACGCTGAGGACTTCATGAATGCTGTTGC
CGTCCAAGGTGATCTGGAACTGCCGGAACTGGCAGAGAAGCTGCAAGGATTCGCAATCGGATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7187.11 Da
• Isoelectric Point: 11.0635

>AT81260 WP_096312685.1 NZ_CP023435:905110-905307 [Mycobacterium sp. PYR15]
MPALNINFSDAEMEQLRTAAAAGEQSLRAFVHRAALDAASDHKRRVREGARIVAERSAELNRRLA

Nucleotide

• Length: 198 bp

>AT81260 NZ_CP023435:905110-905307 [Mycobacterium sp. PYR15]
ATGCCGGCCTTGAACATCAACTTCAGTGACGCGGAGATGGAGCAGTTGCGCACCGCGGCGGCGGCGGGCGAGCAGTCGTT
GCGCGCATTCGTCCACCGCGCCGCCCTTGATGCCGCGAGCGATCACAAGCGTCGCGTGCGGGAGGGTGCGCGGATCGTCG
CCGAGCGCAGCGCAGAGCTCAACCGCCGGCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A1J0UAG7