Detailed information of TA system TA081257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2372576..2373213
• Replicon: chromosome
• Accession: NZ_CP023431
• Organism: Bacillus velezensis strain SCGB 574

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CLI98_RS12040
• Protein ID: WP_003156187.1
• Coordinates: 2372863..2373213 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CLI98_RS12035
• Protein ID: WP_003156188.1
• Coordinates: 2372576..2372857 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLI98_RS12015	2368941..2369540	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
CLI98_RS12020	2369633..2369998	+	366	WP_022552632.1	holo-ACP synthase	-
CLI98_RS12025	2370163..2371170	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
CLI98_RS12030	2371287..2372456	+	1170	WP_022552633.1	alanine racemase	-
CLI98_RS12035	2372576..2372857	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CLI98_RS12040	2372863..2373213	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CLI98_RS12045	2373331..2374152	+	822	WP_003156182.1	STAS domain-containing protein	-
CLI98_RS12050	2374157..2374522	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
CLI98_RS12055	2374525..2374926	+	402	WP_003156178.1	anti-sigma regulatory factor	-
CLI98_RS12060	2374938..2375945	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
CLI98_RS12065	2376009..2376338	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CLI98_RS12070	2376335..2376817	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
CLI98_RS12075	2376783..2377571	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CLI98_RS12080	2377571..2378173	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T81257 WP_003156187.1 NZ_CP023431:2372863-2373213 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T81257 NZ_CP023431:2372863-2373213 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT81257 WP_003156188.1 NZ_CP023431:2372576-2372857 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT81257 NZ_CP023431:2372576-2372857 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5