81254

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 22606..23743 Replicon plasmid pTT39_p3
Accession NZ_CP023426
Organism Enterococcus faecium strain K60-39

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag CNX66_RS15650 Protein ID WP_001284311.1
Coordinates 22606..23469 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag CNX66_RS15655 Protein ID WP_000301765.1
Coordinates 23471..23743 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNX66_RS15620 18178..19473 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
CNX66_RS15625 19718..19969 + 252 Protein_24 GNAT family N-acetyltransferase -
CNX66_RS15630 20062..20856 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
CNX66_RS15635 21266..21349 + 84 Protein_26 peptide-binding protein -
CNX66_RS15640 21398..21481 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
CNX66_RS15645 21606..22343 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
CNX66_RS15650 22606..23469 - 864 WP_001284311.1 toxin zeta Toxin
CNX66_RS15655 23471..23743 - 273 WP_000301765.1 antitoxin Antitoxin
CNX66_RS15660 23760..23969 - 210 WP_000527318.1 peptide-binding protein -
CNX66_RS15665 24067..24327 - 261 WP_096157813.1 hypothetical protein -
CNX66_RS15670 24369..25049 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
CNX66_RS15675 25216..26478 + 1263 WP_000119405.1 plasmid recombination protein -
CNX66_RS15680 26723..27622 + 900 WP_025192414.1 protein rep -
CNX66_RS15685 27645..28325 - 681 WP_002328852.1 IS6-like element IS1216 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid lsa(E) / lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 1..40017 40017
- inside IScluster/Tn lsa(E) / lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 1875..39982 38107


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T81254 WP_001284311.1 NZ_CP023426:c23469-22606 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T81254 NZ_CP023426:c23469-22606 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT81254 WP_000301765.1 NZ_CP023426:c23743-23471 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT81254 NZ_CP023426:c23743-23471 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References