81253

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 14158..15266 Replicon plasmid pTT39_p3
Accession NZ_CP023426
Organism Enterococcus faecium strain K60-39

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag CNX66_RS15595 Protein ID WP_002303392.1
Coordinates 14397..15266 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag CNX66_RS15590 Protein ID WP_002303393.1
Coordinates 14158..14382 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNX66_RS15570 9273..9722 + 450 WP_096157811.1 DNA recombinase -
CNX66_RS15575 10165..11649 + 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -
CNX66_RS15580 11703..12506 + 804 Protein_15 lincosamide nucleotidyltransferase Lnu(B) -
CNX66_RS15585 13194..14015 + 822 Protein_16 recombinase zinc beta ribbon domain-containing protein -
CNX66_RS15590 14158..14382 + 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
CNX66_RS15595 14397..15266 + 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
CNX66_RS15600 15247..15981 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
CNX66_RS15605 16014..16922 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
CNX66_RS15610 16919..17167 + 249 WP_002347175.1 hypothetical protein -
CNX66_RS15620 18178..19473 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
CNX66_RS15625 19718..19969 + 252 Protein_24 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid lsa(E) / lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 1..40017 40017
- inside IScluster/Tn lsa(E) / lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 1875..39982 38107


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T81253 WP_002303392.1 NZ_CP023426:14397-15266 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T81253 NZ_CP023426:14397-15266 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATTGTATTAGGGGGCTC
ACGTGCAAGAAGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAGTTGGATGATGAGAATAGAAACAACCTTGTTGTACCTCCCGGAGCGTGGGGTGATTGG
GTTAATGGCGGTGGATGGTTAGTTATAAACGGGTGTCATGTTGACTTGATATTACGTGATATAAAACGGGTGGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCTATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGCAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGACATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT81253 WP_002303393.1 NZ_CP023426:14158-14382 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT81253 NZ_CP023426:14158-14382 [Enterococcus faecium]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTTAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGATGAAATAAAAATATCTAACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References