Detailed information of TA system TA081252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 52921..53440
• Replicon: plasmid pTT39_p2
• Accession: NZ_CP023425
• Organism: Enterococcus faecium strain K60-39

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: CNX66_RS15320
• Protein ID: WP_000588503.1
• Coordinates: 53183..53440 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: CNX66_RS15315
• Protein ID: WP_000388479.1
• Coordinates: 52921..53190 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNX66_RS15260	48118..49017	+	900	WP_002303312.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
CNX66_RS15265	49020..49505	+	486	WP_002303313.1	single-stranded DNA-binding protein	-
CNX66_RS15270	49646..49849	-	204	WP_002316074.1	putative holin-like toxin	-
CNX66_RS15275	49924..50184	+	261	WP_002295623.1	hypothetical protein	-
CNX66_RS15285	50621..50827	+	207	WP_002347218.1	hypothetical protein	-
CNX66_RS15290	50827..51078	+	252	WP_002296238.1	hypothetical protein	-
CNX66_RS15295	51093..51530	+	438	WP_002300851.1	hypothetical protein	-
CNX66_RS15300	51523..52230	+	708	WP_002303117.1	hypothetical protein	-
CNX66_RS15310	52611..52790	+	180	WP_001120991.1	hypothetical protein	-
CNX66_RS15315	52921..53190	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CNX66_RS15320	53183..53440	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
CNX66_RS16020	53899..54054	+	156	WP_002347142.1	hypothetical protein	-
CNX66_RS15325	54078..54902	+	825	WP_001809248.1	hypothetical protein	-
CNX66_RS15330	55067..55681	-	615	WP_001261742.1	recombinase family protein	-
CNX66_RS15335	55919..56341	+	423	WP_002303116.1	hypothetical protein	-
CNX66_RS15340	56351..56554	+	204	WP_002299573.1	hypothetical protein	-
CNX66_RS15345	56766..57350	+	585	WP_002299575.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86216	86216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T81252 WP_000588503.1 NZ_CP023425:53183-53440 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T81252 NZ_CP023425:53183-53440 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT81252 WP_000388479.1 NZ_CP023425:52921-53190 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT81252 NZ_CP023425:52921-53190 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7