Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 128308..129218 Replicon plasmid pKp1050-1
Accession NZ_CP023417
Organism Klebsiella pneumoniae strain 1050

Toxin (Protein)


Gene name - Uniprot ID A0A2N4Z2R0
Locus tag CN260_RS27615 Protein ID WP_004197882.1
Coordinates 128748..129218 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag CN260_RS27610 Protein ID WP_004181895.1
Coordinates 128308..128751 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CN260_RS27565 124089..124436 - 348 WP_004114612.1 IS66 family insertion sequence element accessory protein TnpB -
CN260_RS27570 124433..124771 - 339 WP_135733211.1 transposase -
CN260_RS27575 124864..125322 + 459 Protein_127 DDE-type integrase/transposase/recombinase -
CN260_RS27580 125527..125838 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin -
CN260_RS27585 125835..126254 + 420 WP_011251286.1 helix-turn-helix domain-containing protein -
CN260_RS27595 126401..127367 + 967 Protein_130 IS5 family transposase -
CN260_RS27600 127401..127595 + 195 WP_110570734.1 DUF2188 domain-containing protein -
CN260_RS27605 127695..128207 + 513 Protein_132 DUF4113 domain-containing protein -
CN260_RS27610 128308..128751 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
CN260_RS27615 128748..129218 + 471 WP_004197882.1 RES family NAD+ phosphorylase Toxin
CN260_RS27620 129329..129589 - 261 WP_004026352.1 hypothetical protein -
CN260_RS27625 130151..131332 - 1182 WP_004883380.1 IS481 family transposase -
CN260_RS27630 131404..131562 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
CN260_RS31545 132168..133142 + 975 WP_174232731.1 Hok/Gef family protein -
CN260_RS27640 133340..133717 - 378 WP_045289277.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid formA pla 1..311641 311641
- inside IScluster/Tn - - 119749..131332 11583


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17633.92 Da        Isoelectric Point: 4.5152

>T81246 WP_004197882.1 NZ_CP023417:128748-129218 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDFFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T81246 NZ_CP023417:128748-129218 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTTTTTTACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCTCTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATTGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT81246 WP_004181895.1 NZ_CP023417:128308-128751 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT81246 NZ_CP023417:128308-128751 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4Z2R0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References