Detailed information of TA system TA081244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 37923..38566
• Replicon: plasmid pKp1050-1
• Accession: NZ_CP023417
• Organism: Klebsiella pneumoniae strain 1050

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CN260_RS27110
• Protein ID: WP_016946796.1
• Coordinates: 38150..38566 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: CN260_RS27105
• Protein ID: WP_110570691.1
• Coordinates: 37923..38153 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CN260_RS27095	33929..36124	+	2196	WP_110570690.1	DEAD/DEAH box helicase	-
CN260_RS27100	36444..37604	-	1161	WP_064171855.1	IS30 family transposase	-
CN260_RS27105	37923..38153	+	231	WP_110570691.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CN260_RS27110	38150..38566	+	417	WP_016946796.1	type II toxin-antitoxin system VapC family toxin	Toxin
CN260_RS27120	38661..39888	+	1228	Protein_40	IS3 family transposase	-
CN260_RS27125	40299..41372	+	1074	WP_110570692.1	DUF4297 domain-containing protein	-
CN260_RS27130	41369..43033	+	1665	WP_110570693.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	pla	1..311641	311641
	-	inside	IScluster/Tn	-	-	36444..49083	12639

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15064.56 Da
• Isoelectric Point: 8.5403

>T81244 WP_016946796.1 NZ_CP023417:38150-38566 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T81244 NZ_CP023417:38150-38566 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGCGCC
GCGGTGGATGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCGGCGCAGTCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8720.76 Da
• Isoelectric Point: 4.4563

>AT81244 WP_110570691.1 NZ_CP023417:37923-38153 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSNEGRFEP

Nucleotide

• Length: 231 bp

>AT81244 NZ_CP023417:37923-38153 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCAATGAAGGACGCTTTGAGCCATGA