Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4041508..4042127 Replicon chromosome
Accession NZ_CP023416
Organism Klebsiella pneumoniae strain 1050

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CN260_RS20455 Protein ID WP_002892050.1
Coordinates 4041909..4042127 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CN260_RS20450 Protein ID WP_002892066.1
Coordinates 4041508..4041882 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CN260_RS20440 4036660..4037853 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CN260_RS20445 4037876..4041022 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CN260_RS20450 4041508..4041882 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CN260_RS20455 4041909..4042127 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CN260_RS20460 4042286..4042852 + 567 WP_002892030.1 maltose O-acetyltransferase -
CN260_RS31425 4042824..4042964 - 141 WP_032410934.1 hypothetical protein -
CN260_RS20465 4042985..4043455 + 471 WP_002892026.1 YlaC family protein -
CN260_RS20470 4043430..4044881 - 1452 WP_029602518.1 PLP-dependent aminotransferase family protein -
CN260_RS20475 4044982..4045680 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
CN260_RS20480 4045677..4045817 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CN260_RS20485 4045817..4046080 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T81239 WP_002892050.1 NZ_CP023416:4041909-4042127 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T81239 NZ_CP023416:4041909-4042127 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT81239 WP_002892066.1 NZ_CP023416:4041508-4041882 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT81239 NZ_CP023416:4041508-4041882 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References