Detailed information of TA system TA081224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2913830..2914467
• Replicon: chromosome
• Accession: NZ_CP023414
• Organism: Bacillus velezensis strain BS-37

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CMR26_RS14415
• Protein ID: WP_003156187.1
• Coordinates: 2913830..2914180 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CMR26_RS14420
• Protein ID: WP_003156188.1
• Coordinates: 2914186..2914467 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CMR26_RS14375	2908867..2909469	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
CMR26_RS14380	2909469..2910257	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CMR26_RS14385	2910223..2910705	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
CMR26_RS14390	2910702..2911031	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CMR26_RS14395	2911095..2912102	-	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
CMR26_RS14400	2912114..2912515	-	402	WP_003156178.1	anti-sigma regulatory factor	-
CMR26_RS14405	2912518..2912883	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
CMR26_RS14410	2912888..2913709	-	822	WP_043021435.1	STAS domain-containing protein	-
CMR26_RS14415	2913830..2914180	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CMR26_RS14420	2914186..2914467	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CMR26_RS14425	2914587..2915756	-	1170	WP_043021434.1	alanine racemase	-
CMR26_RS14430	2915873..2916880	-	1008	WP_043021433.1	outer membrane lipoprotein carrier protein LolA	-
CMR26_RS14435	2917045..2917410	-	366	WP_007410229.1	holo-ACP synthase	-
CMR26_RS14440	2917503..2918102	+	600	WP_020953896.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T81224 WP_003156187.1 NZ_CP023414:c2914180-2913830 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T81224 NZ_CP023414:c2914180-2913830 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT81224 WP_003156188.1 NZ_CP023414:c2914467-2914186 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT81224 NZ_CP023414:c2914467-2914186 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5