Detailed information of TA system TA081201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 2188683..2189130
• Replicon: chromosome
• Accession: NZ_CP023392
• Organism: Lactococcus raffinolactis strain WiKim0068

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CMV25_RS10780
• Protein ID: WP_096040473.1
• Coordinates: 2188864..2189130 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2A5SDT8
• Locus tag: CMV25_RS10775
• Protein ID: WP_061774359.1
• Coordinates: 2188683..2188874 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CMV25_RS10765	2183908..2185863	-	1956	WP_096040471.1	DNA topoisomerase IV subunit B	-
CMV25_RS10770	2186264..2188417	-	2154	WP_096040472.1	type I DNA topoisomerase	-
CMV25_RS10775	2188683..2188874	+	192	WP_061774359.1	hypothetical protein	Antitoxin
CMV25_RS10780	2188864..2189130	+	267	WP_096040473.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CMV25_RS10785	2189199..2190062	-	864	WP_096040474.1	ROK family protein	-
CMV25_RS10790	2190068..2191492	-	1425	WP_096040475.1	glycoside hydrolase family 1 protein	-
CMV25_RS10795	2191707..2192402	-	696	WP_061774362.1	LrgB family protein	-
CMV25_RS10800	2192399..2192761	-	363	WP_061774363.1	CidA/LrgA family protein	-
CMV25_RS10805	2192889..2194100	-	1212	WP_096040552.1	CapA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-87)

Antitoxin

• Protein of unknown function DUF6290 (2-37)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10556.21 Da
• Isoelectric Point: 10.0256

>T81201 WP_096040473.1 NZ_CP023392:2188864-2189130 [Lactococcus raffinolactis]
VTYDFIPSPKFNRQFRILDKFTQKCIQNYINNVILKADNPHNHGKQLVGDLAVLWRYRIGDYRLIVTIQDNELMVPALQI
NHRRDIYR

Nucleotide

• Length: 267 bp

>T81201 NZ_CP023392:2188864-2189130 [Lactococcus raffinolactis]
GTGACGTATGACTTTATCCCTTCGCCTAAATTTAACCGACAATTTCGTATACTTGATAAGTTTACCCAAAAATGCATTCA
AAACTATATTAACAATGTCATCTTAAAAGCAGATAATCCGCACAACCACGGCAAGCAACTTGTTGGAGACTTAGCAGTAC
TTTGGCGTTATCGAATTGGCGACTATCGATTGATAGTCACGATACAGGATAACGAACTGATGGTCCCTGCCTTGCAAATC
AATCATAGACGAGATATCTATCGATAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7328.24 Da
• Isoelectric Point: 4.1683

>AT81201 WP_061774359.1 NZ_CP023392:2188683-2188874 [Lactococcus raffinolactis]
MAKFEGLSLSESIKRTMVEKYENDYDLKVAETSIAEFQKYIISDKPVQSWEDLQPELGLDRDV

Nucleotide

• Length: 192 bp

>AT81201 NZ_CP023392:2188683-2188874 [Lactococcus raffinolactis]
ATGGCGAAATTTGAGGGACTTTCATTATCTGAGAGTATCAAACGTACGATGGTTGAAAAATATGAAAACGATTATGATTT
AAAAGTCGCAGAAACTTCAATTGCTGAATTTCAAAAGTACATTATTTCAGATAAACCAGTCCAATCTTGGGAAGACTTAC
AACCAGAATTGGGGCTTGATCGTGACGTATGA

Structures

Antitoxin

• AlphaFold DB: A0A2A5SDT8