Detailed information of TA system TA081169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1947506..1948282
• Replicon: chromosome
• Accession: NZ_CP023390
• Organism: Staphylococcus aureus subsp. aureus str. Newman strain NYU_Newman

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: CNH35_RS10030
• Protein ID: WP_000031108.1
• Coordinates: 1947506..1947658 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: CNH35_RS10035
• Protein ID: WP_001251224.1
• Coordinates: 1947683..1948282 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNH35_RS10005	1943469..1944290	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
CNH35_RS10010	1944753..1946138	-	1386	WP_000116224.1	class II fumarate hydratase	-
CNH35_RS10015	1946334..1946729	-	396	WP_000901021.1	hypothetical protein	-
CNH35_RS10030	1947506..1947658	-	153	WP_000031108.1	hypothetical protein	Toxin
CNH35_RS10035	1947683..1948282	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
CNH35_RS10040	1948441..1948911	-	471	WP_000181398.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
CNH35_RS10045	1948916..1950043	-	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
CNH35_RS10050	1950194..1950916	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
CNH35_RS10055	1950909..1952366	-	1458	WP_000649907.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T81169 WP_000031108.1 NZ_CP023390:c1947658-1947506 [Staphylococcus aureus subsp. aureus str. Newman]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T81169 NZ_CP023390:c1947658-1947506 [Staphylococcus aureus subsp. aureus str. Newman]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT81169 WP_001251224.1 NZ_CP023390:c1948282-1947683 [Staphylococcus aureus subsp. aureus str. Newman]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT81169 NZ_CP023390:c1948282-1947683 [Staphylococcus aureus subsp. aureus str. Newman]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4