81158

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4588295..4588792 Replicon chromosome
Accession NZ_CP023388
Organism Escherichia coli strain 1105

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CNQ47_RS23560 Protein ID WP_001173343.1
Coordinates 4588295..4588468 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag CNQ47_RS23565 Protein ID WP_001312331.1
Coordinates 4588496..4588792 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ47_RS23550 4584965..4586422 + 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -
CNQ47_RS23555 4586659..4588176 + 1518 WP_000003805.1 lysine--tRNA ligase -
CNQ47_RS23560 4588295..4588468 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CNQ47_RS23565 4588496..4588792 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CNQ47_RS23570 4589021..4589293 - 273 WP_000405647.1 N-acetyltransferase -
CNQ47_RS23575 4589305..4589535 - 231 WP_001683478.1 (4Fe-4S)-binding protein -
CNQ47_RS23580 4589716..4591347 + 1632 WP_001216466.1 sensor histidine kinase -
CNQ47_RS23585 4591344..4592063 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4560636..4588792 28156


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T81158 WP_001173343.1 NZ_CP023388:c4588468-4588295 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T81158 NZ_CP023388:c4588468-4588295 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT81158 WP_001312331.1 NZ_CP023388:c4588792-4588496 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT81158 NZ_CP023388:c4588792-4588496 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References