Detailed information of TA system TA081156

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4574790..4575587
• Replicon: chromosome
• Accession: NZ_CP023388
• Organism: Escherichia coli strain 1105

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M2EZB2
• Locus tag: CNQ47_RS23495
• Protein ID: WP_000854831.1
• Coordinates: 4575213..4575587 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CNQ47_RS23490
• Protein ID: WP_001536509.1
• Coordinates: 4574790..4575167 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ47_RS23450	4570532..4571416	+	885	WP_001536514.1	50S ribosome-binding GTPase	-
CNQ47_RS23455	4571535..4572212	+	678	WP_016237389.1	hypothetical protein	-
CNQ47_RS23460	4572218..4572421	+	204	WP_016237390.1	DUF905 family protein	-
CNQ47_RS23465	4572541..4573359	+	819	WP_096057995.1	DUF945 domain-containing protein	-
CNQ47_RS23470	4573450..4573935	+	486	WP_096057996.1	antirestriction protein	-
CNQ47_RS23480	4573950..4574426	+	477	WP_001186774.1	RadC family protein	-
CNQ47_RS23485	4574495..4574716	+	222	WP_001552746.1	DUF987 domain-containing protein	-
CNQ47_RS23490	4574790..4575167	+	378	WP_001536509.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CNQ47_RS23495	4575213..4575587	+	375	WP_000854831.1	type IV toxin-antitoxin system toxin CbtA	Toxin
CNQ47_RS23500	4575584..4576075	+	492	WP_001683023.1	hypothetical protein	-
CNQ47_RS23505	4576087..4576284	+	198	WP_021531598.1	DUF957 domain-containing protein	-
CNQ47_RS23510	4576369..4577214	+	846	Protein_4297	DUF4942 domain-containing protein	-
CNQ47_RS23515	4577283..4577678	+	396	WP_001536505.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
CNQ47_RS23520	4577671..4578618	+	948	WP_001536504.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4560636..4588792	28156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14051.18 Da
• Isoelectric Point: 8.4983

>T81156 WP_000854831.1 NZ_CP023388:4575213-4575587 [Escherichia coli]
MKTLPVLPKQAASSRPSPLEIWQILLTRLLDQHYGLTLHDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T81156 NZ_CP023388:4575213-4575587 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCAAGCAAGCGGCCAGTTCACGCCCGTCTCCGCTTGAAATCTGGCAGATACTGCTGAC
CCGGCTGCTGGACCAGCATTACGGCCTCACACTGCATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13876.73 Da
• Isoelectric Point: 6.3139

>AT81156 WP_001536509.1 NZ_CP023388:4574790-4575167 [Escherichia coli]
VSDTFSGITHPDDNYDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADVYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPAVPATTA

Nucleotide

• Length: 378 bp

>AT81156 NZ_CP023388:4574790-4575167 [Escherichia coli]
GTGTCAGACACATTCTCCGGGATAACTCATCCCGACGACAATTACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGTATACCATCTGGACCAAGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTCAGTCCCCGTCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACACCAGCAGTTCCCGCAACAACAGCATAA

Structures

Toxin

• AlphaFold DB: A0A1M2EZB2