Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yeeU/CbtA-CbeA
Location 4331326..4332033 Replicon chromosome
Accession NZ_CP023388
Organism Escherichia coli strain 1105

Toxin (Protein)


Gene name ykfI Uniprot ID A0A4T3Q6D2
Locus tag CNQ47_RS22265 Protein ID WP_001683515.1
Coordinates 4331326..4331667 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3L1KVZ6
Locus tag CNQ47_RS22270 Protein ID WP_000939437.1
Coordinates 4331698..4332033 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ47_RS22245 4328863..4329660 - 798 WP_001552326.1 helix-turn-helix transcriptional regulator -
CNQ47_RS22250 4329774..4330049 - 276 WP_001683517.1 type II toxin-antitoxin system RelE/ParE family toxin -
CNQ47_RS22255 4330053..4330301 - 249 WP_024187601.1 ribbon-helix-helix domain-containing protein -
CNQ47_RS22260 4330378..4331211 - 834 WP_001683516.1 DUF4942 domain-containing protein -
CNQ47_RS22265 4331326..4331667 - 342 WP_001683515.1 TA system toxin CbtA family protein Toxin
CNQ47_RS22270 4331698..4332033 - 336 WP_000939437.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CNQ47_RS22275 4332033..4332506 - 474 WP_001296921.1 DNA repair protein RadC -
CNQ47_RS22280 4332536..4333354 - 819 WP_001683514.1 DUF945 domain-containing protein -
CNQ47_RS22285 4333590..4334543 - 954 WP_001683513.1 hypothetical protein -
CNQ47_RS22290 4335136..4335750 + 615 WP_001683512.1 inovirus Gp2 family protein -
CNQ47_RS22295 4335868..4336086 + 219 WP_001683511.1 AlpA family phage regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB 4319345..4347353 28008


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-110)

Antitoxin

Antitoxin CbeA

(10-110)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13034.05 Da        Isoelectric Point: 9.5454

>T81155 WP_001683515.1 NZ_CP023388:c4331667-4331326 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCHNTTAR

Download         Length: 342 bp

>T81155 NZ_CP023388:c4331667-4331326 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGACTGAACCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTACGGGCGCGACAGGCTACAGGTCTGCTGCGCCGCTG
CCATAACACCACGGCACGGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12556.56 Da        Isoelectric Point: 7.6977

>AT81155 WP_000939437.1 NZ_CP023388:c4332033-4331698 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 336 bp

>AT81155 NZ_CP023388:c4332033-4331698 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCTCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4T3Q6D2

Antitoxin

Source ID Structure
AlphaFold DB A0A3L1KVZ6

References