Detailed information of TA system TA081152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4140753..4141303
• Replicon: chromosome
• Accession: NZ_CP023388
• Organism: Escherichia coli strain 1105

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A8H8ZN44
• Locus tag: CNQ47_RS21350
• Protein ID: WP_001160969.1
• Coordinates: 4140753..4141067 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: CNQ47_RS21355
• Protein ID: WP_000125566.1
• Coordinates: 4141070..4141303 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ47_RS21325	4136428..4137714	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
CNQ47_RS21330	4137714..4138703	+	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
CNQ47_RS21335	4138700..4139521	+	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
CNQ47_RS21340	4139524..4139901	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
CNQ47_RS21345	4139908..4140756	+	849	WP_000257181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
CNQ47_RS21350	4140753..4141067	-	315	WP_001160969.1	CcdB family protein	Toxin
CNQ47_RS21355	4141070..4141303	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CNQ47_RS21360	4141389..4141868	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
CNQ47_RS21365	4142060..4143922	-	1863	WP_000377124.1	glutathione-regulated potassium-efflux system protein KefC	-
CNQ47_RS21370	4143915..4144445	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
CNQ47_RS21375	4144553..4145884	-	1332	WP_096057983.1	MFS transporter	-
CNQ47_RS21380	4145943..4146230	-	288	WP_001682556.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11681.65 Da
• Isoelectric Point: 9.0851

>T81152 WP_001160969.1 NZ_CP023388:c4141067-4140753 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPIERFSRIRPPERLNPILLPVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T81152 NZ_CP023388:c4141067-4140753 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTGTTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACCGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACAAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT81152 WP_000125566.1 NZ_CP023388:c4141303-4141070 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT81152 NZ_CP023388:c4141303-4141070 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9