Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1138182..1138836 Replicon chromosome
Accession NZ_CP023388
Organism Escherichia coli strain 1105

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CNQ47_RS05980 Protein ID WP_000244781.1
Coordinates 1138429..1138836 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CNQ47_RS05975 Protein ID WP_000354046.1
Coordinates 1138182..1138448 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ47_RS05950 1134260..1135693 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
CNQ47_RS05955 1135738..1136049 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
CNQ47_RS05960 1136213..1136872 + 660 WP_000250274.1 hemolysin III family protein -
CNQ47_RS05965 1136949..1137929 - 981 WP_001683259.1 tRNA-modifying protein YgfZ -
CNQ47_RS05975 1138182..1138448 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CNQ47_RS05980 1138429..1138836 + 408 WP_000244781.1 protein YgfX Toxin
CNQ47_RS05985 1138876..1139397 - 522 WP_001683258.1 flavodoxin FldB -
CNQ47_RS05990 1139509..1140405 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
CNQ47_RS05995 1140430..1141140 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CNQ47_RS06000 1141146..1142879 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T81135 WP_000244781.1 NZ_CP023388:1138429-1138836 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T81135 NZ_CP023388:1138429-1138836 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTAATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTACGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT81135 WP_000354046.1 NZ_CP023388:1138182-1138448 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT81135 NZ_CP023388:1138182-1138448 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References