Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4472164..4472661 Replicon chromosome
Accession NZ_CP023386
Organism Escherichia coli strain 1190

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CNQ48_RS22890 Protein ID WP_001173343.1
Coordinates 4472164..4472337 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2X1N3L1
Locus tag CNQ48_RS22895 Protein ID WP_001560287.1
Coordinates 4472365..4472661 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ48_RS22880 4468834..4470291 + 1458 WP_000856819.1 dipeptide/tripeptide permease DtpC -
CNQ48_RS22885 4470528..4472045 + 1518 WP_000003806.1 lysine--tRNA ligase -
CNQ48_RS22890 4472164..4472337 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CNQ48_RS22895 4472365..4472661 - 297 WP_001560287.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CNQ48_RS22900 4472890..4473162 - 273 WP_000405647.1 N-acetyltransferase -
CNQ48_RS22905 4473174..4473404 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
CNQ48_RS22910 4473585..4475216 + 1632 WP_001216477.1 sensor histidine kinase -
CNQ48_RS22915 4475213..4475932 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T81127 WP_001173343.1 NZ_CP023386:c4472337-4472164 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T81127 NZ_CP023386:c4472337-4472164 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11441.94 Da        Isoelectric Point: 4.2924

>AT81127 WP_001560287.1 NZ_CP023386:c4472661-4472365 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMHDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT81127 NZ_CP023386:c4472661-4472365 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGCATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1N3L1

References