Detailed information of TA system TA081122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4093983..4094470
• Replicon: chromosome
• Accession: NZ_CP023386
• Organism: Escherichia coli strain 1190

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CNQ48_RS20830
• Protein ID: WP_001160961.1
• Coordinates: 4093983..4094234 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: CNQ48_RS20835
• Protein ID: WP_000125566.1
• Coordinates: 4094237..4094470 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ48_RS20805	4089595..4090881	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
CNQ48_RS20810	4090881..4091870	+	990	WP_000241249.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
CNQ48_RS20815	4091867..4092688	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
CNQ48_RS20820	4092691..4093068	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
CNQ48_RS20825	4093075..4093923	+	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
CNQ48_RS20830	4093983..4094234	-	252	WP_001160961.1	CcdB family protein	Toxin
CNQ48_RS20835	4094237..4094470	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CNQ48_RS20840	4094556..4095035	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
CNQ48_RS20845	4095227..4097089	-	1863	WP_000377114.1	glutathione-regulated potassium-efflux system protein KefC	-
CNQ48_RS20850	4097082..4097612	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
CNQ48_RS20855	4097720..4099051	-	1332	WP_001183198.1	MFS transporter	-
CNQ48_RS20860	4099110..4099397	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9512.19 Da
• Isoelectric Point: 10.4134

>T81122 WP_001160961.1 NZ_CP023386:c4094234-4093983 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T81122 NZ_CP023386:c4094234-4093983 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT81122 WP_000125566.1 NZ_CP023386:c4094470-4094237 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT81122 NZ_CP023386:c4094470-4094237 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9