Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3597881..3598499 Replicon chromosome
Accession NZ_CP023386
Organism Escherichia coli strain 1190

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CNQ48_RS18370 Protein ID WP_001291435.1
Coordinates 3598281..3598499 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CNQ48_RS18365 Protein ID WP_000344800.1
Coordinates 3597881..3598255 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ48_RS18355 3592970..3594163 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CNQ48_RS18360 3594186..3597335 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
CNQ48_RS18365 3597881..3598255 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CNQ48_RS18370 3598281..3598499 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CNQ48_RS18375 3598671..3599222 + 552 WP_000102564.1 maltose O-acetyltransferase -
CNQ48_RS18380 3599338..3599808 + 471 WP_000136192.1 YlaC family protein -
CNQ48_RS18385 3599972..3601522 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CNQ48_RS18390 3601564..3601917 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CNQ48_RS18400 3602296..3602607 + 312 WP_000409911.1 MGMT family protein -
CNQ48_RS18405 3602638..3603210 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T81119 WP_001291435.1 NZ_CP023386:3598281-3598499 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T81119 NZ_CP023386:3598281-3598499 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT81119 WP_000344800.1 NZ_CP023386:3597881-3598255 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT81119 NZ_CP023386:3597881-3598255 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References