Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 128130..128773 Replicon plasmid p147
Accession NZ_CP023384
Organism Escherichia coli strain 1223

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag CNQ49_RS25175 Protein ID WP_001034046.1
Coordinates 128357..128773 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag CNQ49_RS25170 Protein ID WP_001261278.1
Coordinates 128130..128360 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ49_RS25135 123642..123947 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
CNQ49_RS25140 123949..124167 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
CNQ49_RS25150 124733..125245 + 513 WP_000151784.1 hypothetical protein -
CNQ49_RS25155 125279..126412 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
CNQ49_RS25160 126579..127352 - 774 WP_000905949.1 hypothetical protein -
CNQ49_RS25165 127365..127865 - 501 WP_000528932.1 hypothetical protein -
CNQ49_RS25170 128130..128360 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CNQ49_RS25175 128357..128773 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
CNQ49_RS25180 128818..132612 - 3795 WP_001144732.1 hypothetical protein -
CNQ49_RS25185 132993..133223 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CNQ49_RS25190 133220..133636 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / iucA 1..147369 147369


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T81099 WP_001034046.1 NZ_CP023384:128357-128773 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T81099 NZ_CP023384:128357-128773 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT81099 WP_001261278.1 NZ_CP023384:128130-128360 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT81099 NZ_CP023384:128130-128360 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References