Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 123642..124167 Replicon plasmid p147
Accession NZ_CP023384
Organism Escherichia coli strain 1223

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CNQ49_RS25135 Protein ID WP_001159871.1
Coordinates 123642..123947 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag CNQ49_RS25140 Protein ID WP_000813630.1
Coordinates 123949..124167 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ49_RS25120 119598..120803 - 1206 WP_001442122.1 AAA family ATPase -
CNQ49_RS25125 121424..122155 + 732 WP_000504262.1 replication initiation protein -
CNQ49_RS25130 122835..123641 - 807 WP_000016968.1 site-specific integrase -
CNQ49_RS25135 123642..123947 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CNQ49_RS25140 123949..124167 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CNQ49_RS25150 124733..125245 + 513 WP_000151784.1 hypothetical protein -
CNQ49_RS25155 125279..126412 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
CNQ49_RS25160 126579..127352 - 774 WP_000905949.1 hypothetical protein -
CNQ49_RS25165 127365..127865 - 501 WP_000528932.1 hypothetical protein -
CNQ49_RS25170 128130..128360 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CNQ49_RS25175 128357..128773 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / iucA 1..147369 147369


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T81098 WP_001159871.1 NZ_CP023384:c123947-123642 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T81098 NZ_CP023384:c123947-123642 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT81098 WP_000813630.1 NZ_CP023384:c124167-123949 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT81098 NZ_CP023384:c124167-123949 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGTACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References