Detailed information of TA system TA081083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3639285..3639868
• Replicon: chromosome
• Accession: NZ_CP023383
• Organism: Escherichia coli strain 1223

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CNQ49_RS18755
• Protein ID: WP_049253269.1
• Coordinates: 3639285..3639620 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: CNQ49_RS18760
• Protein ID: WP_000581941.1
• Coordinates: 3639620..3639868 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ49_RS18740	3635172..3636470	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
CNQ49_RS18745	3636558..3638195	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
CNQ49_RS18750	3638423..3639214	-	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
CNQ49_RS18755	3639285..3639620	-	336	WP_049253269.1	endoribonuclease MazF	Toxin
CNQ49_RS18760	3639620..3639868	-	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CNQ49_RS18765	3639946..3642180	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
CNQ49_RS18770	3642228..3643529	-	1302	WP_023281448.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12197.18 Da
• Isoelectric Point: 8.7218

>T81083 WP_049253269.1 NZ_CP023383:c3639620-3639285 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQARHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T81083 NZ_CP023383:c3639620-3639285 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTCG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT81083 WP_000581941.1 NZ_CP023383:c3639868-3639620 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT81083 NZ_CP023383:c3639868-3639620 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9