Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 59795..60396 Replicon plasmid p91
Accession NZ_CP023381
Organism Escherichia coli strain 127

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag CNQ50_RS26735 Protein ID WP_001216045.1
Coordinates 59795..60175 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CNQ50_RS26740 Protein ID WP_001190712.1
Coordinates 60175..60396 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ50_RS26710 55235..56719 - 1485 WP_096038044.1 terminase -
CNQ50_RS26715 56719..57912 - 1194 WP_096038045.1 terminase -
CNQ50_RS26720 57999..58451 - 453 WP_001312282.1 late promoter-activating protein (Gp10) -
CNQ50_RS26725 58540..59583 - 1044 WP_000648827.1 DUF968 domain-containing protein -
CNQ50_RS26730 59611..59790 - 180 WP_000113019.1 hypothetical protein -
CNQ50_RS26735 59795..60175 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CNQ50_RS26740 60175..60396 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CNQ50_RS26745 60469..60858 - 390 WP_000506726.1 DNA repair protein -
CNQ50_RS26750 60982..61233 - 252 WP_001377386.1 DNA polymerase III subunit theta -
CNQ50_RS28240 61683..61820 + 138 WP_000123562.1 hypothetical protein -
CNQ50_RS26760 61895..62257 - 363 WP_001261544.1 hypothetical protein -
CNQ50_RS26765 62254..63186 - 933 WP_000057449.1 hypothetical protein -
CNQ50_RS26770 63168..63542 - 375 WP_000988658.1 hypothetical protein -
CNQ50_RS26775 63549..63842 - 294 WP_001677496.1 hypothetical protein -
CNQ50_RS26780 64021..64254 - 234 WP_000516537.1 hypothetical protein -
CNQ50_RS26785 64337..65224 - 888 WP_023153863.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..91199 91199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T81060 WP_001216045.1 NZ_CP023381:c60175-59795 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T81060 NZ_CP023381:c60175-59795 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT81060 WP_001190712.1 NZ_CP023381:c60396-60175 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT81060 NZ_CP023381:c60396-60175 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References