Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 39308..39773 Replicon plasmid p43
Accession NZ_CP023380
Organism Escherichia coli strain 127

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag CNQ50_RS26395 Protein ID WP_001230707.1
Coordinates 39522..39773 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag CNQ50_RS26390 Protein ID WP_000801440.1
Coordinates 39308..39535 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ50_RS26340 34715..34930 - 216 WP_000760375.1 EexN family lipoprotein -
CNQ50_RS26345 34927..35220 - 294 WP_096038029.1 LuxR family transcriptional regulator -
CNQ50_RS26350 35297..36526 - 1230 WP_000539543.1 relaxase/mobilization nuclease domain-containing protein -
CNQ50_RS26355 36530..37033 - 504 WP_000781814.1 hypothetical protein -
CNQ50_RS26365 37393..37524 - 132 WP_001353745.1 toxin-antitoxin system HicB family antitoxin -
CNQ50_RS26370 37821..38132 - 312 WP_000627400.1 hypothetical protein -
CNQ50_RS26375 38129..38545 - 417 WP_012421208.1 tail-associated lysozyme -
CNQ50_RS26380 38555..38854 - 300 WP_000650311.1 hypothetical protein -
CNQ50_RS26390 39308..39535 - 228 WP_000801440.1 hypothetical protein Antitoxin
CNQ50_RS26395 39522..39773 - 252 WP_001230707.1 hypothetical protein Toxin
CNQ50_RS26400 39770..40312 - 543 WP_001083906.1 hypothetical protein -
CNQ50_RS28015 40417..40932 - 516 WP_001025395.1 J domain-containing protein -
CNQ50_RS26410 41572..41922 - 351 WP_000127482.1 addiction module antitoxin -
CNQ50_RS26415 41919..42254 - 336 WP_001147119.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-35 / dfrA1 - 1..43592 43592


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T81059 WP_001230707.1 NZ_CP023380:c39773-39522 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T81059 NZ_CP023380:c39773-39522 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT81059 WP_000801440.1 NZ_CP023380:c39535-39308 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT81059 NZ_CP023380:c39535-39308 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References