Detailed information of TA system TA081045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3143702..3144407
• Replicon: chromosome
• Accession: NZ_CP023377
• Organism: Escherichia coli strain 127

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: CNQ50_RS16835
• Protein ID: WP_000539521.1
• Coordinates: 3143702..3144088 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CNQ50_RS16840
• Protein ID: WP_001280945.1
• Coordinates: 3144078..3144407 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ50_RS16815	3139706..3140332	+	627	WP_001295292.1	glutathione S-transferase GstB	-
CNQ50_RS16820	3140329..3141444	-	1116	Protein_3083	aldose sugar dehydrogenase YliI	-
CNQ50_RS16825	3141555..3141938	-	384	WP_000497137.1	biofilm formation regulator BssR	-
CNQ50_RS16830	3142151..3143476	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CNQ50_RS16835	3143702..3144088	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CNQ50_RS16840	3144078..3144407	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CNQ50_RS16845	3144477..3145805	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
CNQ50_RS16850	3145813..3148161	-	2349	WP_096038021.1	EAL domain-containing protein	-
CNQ50_RS16855	3148339..3149250	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T81045 WP_000539521.1 NZ_CP023377:3143702-3144088 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T81045 NZ_CP023377:3143702-3144088 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT81045 WP_001280945.1 NZ_CP023377:3144078-3144407 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT81045 NZ_CP023377:3144078-3144407 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATAA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6