Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2442059..2442697 Replicon chromosome
Accession NZ_CP023377
Organism Escherichia coli strain 127

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CNQ50_RS12825 Protein ID WP_000813794.1
Coordinates 2442521..2442697 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CNQ50_RS12820 Protein ID WP_001270286.1
Coordinates 2442059..2442475 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ50_RS12800 2437211..2438152 - 942 WP_001251313.1 ABC transporter permease -
CNQ50_RS12805 2438153..2439166 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
CNQ50_RS12810 2439184..2440329 - 1146 WP_096037851.1 ABC transporter substrate-binding protein -
CNQ50_RS12815 2440574..2441980 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
CNQ50_RS12820 2442059..2442475 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CNQ50_RS12825 2442521..2442697 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CNQ50_RS12830 2442919..2443149 + 231 WP_000494244.1 YncJ family protein -
CNQ50_RS12835 2443241..2445202 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CNQ50_RS12840 2445275..2445811 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
CNQ50_RS12845 2445864..2447078 + 1215 WP_071607224.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T81044 WP_000813794.1 NZ_CP023377:c2442697-2442521 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T81044 NZ_CP023377:c2442697-2442521 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT81044 WP_001270286.1 NZ_CP023377:c2442475-2442059 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT81044 NZ_CP023377:c2442475-2442059 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References