Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3995416..3995992 Replicon chromosome
Accession NZ_CP023371
Organism Escherichia coli strain 1283

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag CNQ51_RS20565 Protein ID WP_001297643.1
Coordinates 3995705..3995992 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag CNQ51_RS20560 Protein ID WP_000063156.1
Coordinates 3995416..3995718 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ51_RS20545 3992012..3994162 + 2151 WP_001446922.1 pyruvate/proton symporter BtsT -
CNQ51_RS20550 3994212..3994415 + 204 WP_000467859.1 YbdD/YjiX family protein -
CNQ51_RS20555 3994426..3995382 + 957 WP_001297640.1 GTPase -
CNQ51_RS20560 3995416..3995718 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
CNQ51_RS20565 3995705..3995992 - 288 WP_001297643.1 BrnT family toxin Toxin
CNQ51_RS20570 3996191..3996888 - 698 Protein_3764 IS1 family transposase -
CNQ51_RS20575 3996945..3997040 + 96 Protein_3765 dienelactone hydrolase family protein -
CNQ51_RS20580 3997186..3998418 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
CNQ51_RS20585 3998459..3999739 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T81014 WP_001297643.1 NZ_CP023371:c3995992-3995705 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T81014 NZ_CP023371:c3995992-3995705 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT81014 WP_000063156.1 NZ_CP023371:c3995718-3995416 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT81014 NZ_CP023371:c3995718-3995416 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References