Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 79966..80513 Replicon plasmid p96
Accession NZ_CP023370
Organism Escherichia coli strain 1428

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag CNQ52_RS27480 Protein ID WP_000969996.1
Coordinates 80232..80513 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag CNQ52_RS27475 Protein ID WP_000079928.1
Coordinates 79966..80235 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ52_RS27430 75610..76143 - 534 WP_001303319.1 transcription termination factor NusG -
CNQ52_RS27440 76585..76872 - 288 WP_000074849.1 conjugal transfer protein TraA -
CNQ52_RS27445 76790..77053 - 264 WP_001324596.1 ash family protein -
CNQ52_RS27460 78026..79057 + 1032 WP_000907873.1 replication initiation protein -
CNQ52_RS27475 79966..80235 + 270 WP_000079928.1 hypothetical protein Antitoxin
CNQ52_RS27480 80232..80513 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CNQ52_RS27485 80553..81401 + 849 WP_001057996.1 3'-5' exonuclease -
CNQ52_RS27490 81518..81991 + 474 WP_001334658.1 hypothetical protein -
CNQ52_RS27505 82734..83228 - 495 WP_023278058.1 hypothetical protein -
CNQ52_RS27510 83284..83886 - 603 WP_000517689.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-2 - 1..96402 96402


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T80994 WP_000969996.1 NZ_CP023370:80232-80513 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T80994 NZ_CP023370:80232-80513 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT80994 WP_000079928.1 NZ_CP023370:79966-80235 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT80994 NZ_CP023370:79966-80235 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References