Detailed information of TA system TA080989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1786..2333
• Replicon: plasmid p66
• Accession: NZ_CP023369
• Organism: Escherichia coli strain 1428

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: CNQ52_RS26425
• Protein ID: WP_000969996.1
• Coordinates: 2052..2333 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q4FIL6
• Locus tag: CNQ52_RS26420
• Protein ID: WP_000079945.1
• Coordinates: 1786..2055 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ52_RS26405	13..870	+	858	WP_000130995.1	incFII family plasmid replication initiator RepA	-
CNQ52_RS27935	1573..1713	+	141	WP_000829596.1	hypothetical protein	-
CNQ52_RS26420	1786..2055	+	270	WP_000079945.1	hypothetical protein	Antitoxin
CNQ52_RS26425	2052..2333	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CNQ52_RS28060	2405..2583	+	179	Protein_5	type II toxin-antitoxin system RelE/ParE family toxin	-
CNQ52_RS26435	2654..3535	+	882	WP_000005460.1	hypothetical protein	-
CNQ52_RS26440	3470..3889	-	420	WP_024181414.1	hypothetical protein	-
CNQ52_RS26445	3998..4348	-	351	WP_001414994.1	plasmid partitioning/stability family protein	-
CNQ52_RS26450	4351..5313	-	963	WP_000959884.1	plasmid segregation protein ParM	-
CNQ52_RS26460	5848..6750	+	903	WP_001513647.1	DUF1281 domain-containing protein	-
CNQ52_RS26465	6747..7058	+	312	WP_032144809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B)	-	1..66332	66332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T80989 WP_000969996.1 NZ_CP023369:2052-2333 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T80989 NZ_CP023369:2052-2333 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGAAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10180.52 Da
• Isoelectric Point: 8.8735

>AT80989 WP_000079945.1 NZ_CP023369:1786-2055 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT80989 NZ_CP023369:1786-2055 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTTCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: Q4FIL6