Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 59220..59745 Replicon plasmid p111
Accession NZ_CP023367
Organism Escherichia coli strain 1428

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CNQ52_RS25690 Protein ID WP_001159871.1
Coordinates 59440..59745 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag CNQ52_RS25685 Protein ID WP_000813639.1
Coordinates 59220..59438 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ52_RS25665 55415..56005 - 591 WP_001595311.1 hypothetical protein -
CNQ52_RS25670 56005..56262 - 258 WP_000343099.1 hypothetical protein -
CNQ52_RS25675 56582..57514 - 933 WP_001595310.1 hypothetical protein -
CNQ52_RS25680 57518..58513 - 996 WP_001595309.1 hypothetical protein -
CNQ52_RS25685 59220..59438 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CNQ52_RS25690 59440..59745 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CNQ52_RS25695 59746..60555 + 810 WP_001595307.1 site-specific integrase -
CNQ52_RS25700 60693..60968 - 276 WP_000239529.1 hypothetical protein -
CNQ52_RS25705 60962..61606 - 645 WP_000633911.1 AAA family ATPase -
CNQ52_RS25710 61835..62806 + 972 WP_001103690.1 hypothetical protein -
CNQ52_RS25715 62811..63203 + 393 WP_001595306.1 plasmid partitioning/stability family protein -
CNQ52_RS25720 63208..64480 - 1273 Protein_62 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iroN / iroE / iroD / iroC / iroB 1..111523 111523


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T80987 WP_001159871.1 NZ_CP023367:59440-59745 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T80987 NZ_CP023367:59440-59745 [Escherichia coli]
ATGCAGTTTAAAGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTC
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT80987 WP_000813639.1 NZ_CP023367:59220-59438 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT80987 NZ_CP023367:59220-59438 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References