Detailed information of TA system TA080981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4367854..4368449
• Replicon: chromosome
• Accession: NZ_CP023366
• Organism: Escherichia coli strain 1428

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: CNQ52_RS22105
• Protein ID: WP_000239581.1
• Coordinates: 4367854..4368204 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: CNQ52_RS22110
• Protein ID: WP_001223213.1
• Coordinates: 4368198..4368449 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ52_RS22085	4363308..4364330	-	1023	WP_001298067.1	ABC transporter permease	-
CNQ52_RS22090	4364344..4365846	-	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
CNQ52_RS22095	4365979..4366935	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
CNQ52_RS22100	4367245..4367775	+	531	WP_000055075.1	inorganic diphosphatase	-
CNQ52_RS22105	4367854..4368204	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
CNQ52_RS22110	4368198..4368449	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
CNQ52_RS22115	4368661..4369002	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
CNQ52_RS22120	4369005..4372784	-	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T80981 WP_000239581.1 NZ_CP023366:c4368204-4367854 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T80981 NZ_CP023366:c4368204-4367854 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT80981 WP_001223213.1 NZ_CP023366:c4368449-4368198 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT80981 NZ_CP023366:c4368449-4368198 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7