Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4083560..4084110 Replicon chromosome
Accession NZ_CP023366
Organism Escherichia coli strain 1428

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag CNQ52_RS20595 Protein ID WP_001160966.1
Coordinates 4083560..4083874 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag CNQ52_RS20600 Protein ID WP_000125568.1
Coordinates 4083877..4084110 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ52_RS20570 4079235..4080521 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
CNQ52_RS20575 4080521..4081510 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CNQ52_RS20580 4081507..4082328 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CNQ52_RS20585 4082331..4082708 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CNQ52_RS20590 4082715..4083563 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CNQ52_RS20595 4083560..4083874 - 315 WP_001160966.1 CcdB family protein Toxin
CNQ52_RS20600 4083877..4084110 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CNQ52_RS20605 4084196..4084675 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
CNQ52_RS20610 4084867..4086729 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
CNQ52_RS20615 4086722..4087252 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CNQ52_RS20620 4087360..4088691 - 1332 WP_001183201.1 MFS transporter -
CNQ52_RS20625 4088749..4089036 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T80975 WP_001160966.1 NZ_CP023366:c4083874-4083560 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T80975 NZ_CP023366:c4083874-4083560 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCACACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT80975 WP_000125568.1 NZ_CP023366:c4084110-4083877 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT80975 NZ_CP023366:c4084110-4083877 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References