Detailed information of TA system TA080971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3224269..3224974
• Replicon: chromosome
• Accession: NZ_CP023366
• Organism: Escherichia coli strain 1428

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: CNQ52_RS16225
• Protein ID: WP_000539521.1
• Coordinates: 3224269..3224655 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CNQ52_RS16230
• Protein ID: WP_001280945.1
• Coordinates: 3224645..3224974 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ52_RS16205	3220273..3220899	+	627	WP_001295292.1	glutathione S-transferase GstB	-
CNQ52_RS16210	3220896..3222011	-	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
CNQ52_RS16215	3222122..3222505	-	384	WP_000497137.1	biofilm formation regulator BssR	-
CNQ52_RS16220	3222718..3224043	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CNQ52_RS16225	3224269..3224655	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CNQ52_RS16230	3224645..3224974	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CNQ52_RS16235	3225044..3226372	-	1329	WP_022645414.1	GGDEF domain-containing protein	-
CNQ52_RS16240	3226380..3228728	-	2349	WP_096040729.1	EAL domain-containing protein	-
CNQ52_RS16245	3228905..3229816	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T80971 WP_000539521.1 NZ_CP023366:3224269-3224655 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T80971 NZ_CP023366:3224269-3224655 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT80971 WP_001280945.1 NZ_CP023366:3224645-3224974 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT80971 NZ_CP023366:3224645-3224974 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6