Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 212291..212903 Replicon chromosome
Accession NZ_CP023366
Organism Escherichia coli strain 1428

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag CNQ52_RS00970 Protein ID WP_000833473.1
Coordinates 212291..212476 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0A1AGA0
Locus tag CNQ52_RS00975 Protein ID WP_022646255.1
Coordinates 212493..212903 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ52_RS00950 207757..209052 + 1296 WP_000985736.1 Fic family protein -
CNQ52_RS00955 209160..210698 + 1539 WP_000183976.1 aldehyde dehydrogenase AldB -
CNQ52_RS00960 210739..211818 - 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
CNQ52_RS00970 212291..212476 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
CNQ52_RS00975 212493..212903 + 411 WP_022646255.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CNQ52_RS00980 213015..214994 - 1980 WP_022646254.1 glycoside hydrolase family 127 protein -
CNQ52_RS00985 215005..216405 - 1401 WP_001600688.1 MFS transporter -
CNQ52_RS00990 216631..217446 + 816 WP_022646253.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T80955 WP_000833473.1 NZ_CP023366:212291-212476 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T80955 NZ_CP023366:212291-212476 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15214.08 Da        Isoelectric Point: 4.4482

>AT80955 WP_022646255.1 NZ_CP023366:212493-212903 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALAAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT80955 NZ_CP023366:212493-212903 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGCCGCGCACTTCGAAACCTTATGCGAGATGG
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AGA0

References