Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3754680..3755298 Replicon chromosome
Accession NZ_CP023364
Organism Escherichia coli strain 144

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CNQ53_RS19875 Protein ID WP_001291435.1
Coordinates 3755080..3755298 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CNQ53_RS19870 Protein ID WP_000344800.1
Coordinates 3754680..3755054 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ53_RS19860 3749769..3750962 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CNQ53_RS19865 3750985..3754134 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
CNQ53_RS19870 3754680..3755054 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CNQ53_RS19875 3755080..3755298 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CNQ53_RS19880 3755470..3756021 + 552 WP_000102564.1 maltose O-acetyltransferase -
CNQ53_RS19885 3756137..3756607 + 471 WP_000136192.1 YlaC family protein -
CNQ53_RS19890 3756771..3758321 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CNQ53_RS19895 3758363..3758716 - 354 WP_001550741.1 DUF1428 family protein -
CNQ53_RS19905 3759095..3759406 + 312 WP_000409911.1 MGMT family protein -
CNQ53_RS19910 3759437..3760009 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T80943 WP_001291435.1 NZ_CP023364:3755080-3755298 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T80943 NZ_CP023364:3755080-3755298 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT80943 WP_000344800.1 NZ_CP023364:3754680-3755054 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT80943 NZ_CP023364:3754680-3755054 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References