Detailed information of TA system TA080940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2660226..2660864
• Replicon: chromosome
• Accession: NZ_CP023364
• Organism: Escherichia coli strain 144

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: CNQ53_RS14255
• Protein ID: WP_000813795.1
• Coordinates: 2660688..2660864 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CNQ53_RS14250
• Protein ID: WP_076797675.1
• Coordinates: 2660226..2660642 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ53_RS14230	2655378..2656319	-	942	WP_001251315.1	ABC transporter permease	-
CNQ53_RS14235	2656320..2657333	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
CNQ53_RS14240	2657351..2658496	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
CNQ53_RS14245	2658741..2660147	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
CNQ53_RS14250	2660226..2660642	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CNQ53_RS14255	2660688..2660864	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CNQ53_RS14260	2661086..2661316	+	231	WP_023910283.1	YncJ family protein	-
CNQ53_RS14265	2661408..2663369	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CNQ53_RS14270	2663442..2663978	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
CNQ53_RS14275	2664031..2665242	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T80940 WP_000813795.1 NZ_CP023364:c2660864-2660688 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T80940 NZ_CP023364:c2660864-2660688 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT80940 WP_076797675.1 NZ_CP023364:c2660642-2660226 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT80940 NZ_CP023364:c2660642-2660226 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1