Detailed information of TA system TA080927

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 875359..876194
• Replicon: chromosome
• Accession: NZ_CP023364
• Organism: Escherichia coli strain 144

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: CNQ53_RS05315
• Protein ID: WP_001094445.1
• Coordinates: 875359..875736 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CNQ53_RS05320
• Protein ID: WP_024174331.1
• Coordinates: 875826..876194 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ53_RS05290	871431..872414	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
CNQ53_RS05295	873225..873380	-	156	WP_000729638.1	hypothetical protein	-
CNQ53_RS05300	873738..874580	-	843	WP_001280499.1	DUF4942 domain-containing protein	-
CNQ53_RS05305	874665..874862	-	198	WP_000838536.1	DUF957 domain-containing protein	-
CNQ53_RS05310	874874..875362	-	489	WP_000761717.1	hypothetical protein	-
CNQ53_RS05315	875359..875736	-	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
CNQ53_RS05320	875826..876194	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CNQ53_RS05325	876268..876489	-	222	WP_000692346.1	DUF987 domain-containing protein	-
CNQ53_RS05330	876558..877034	-	477	WP_001186725.1	RadC family protein	-
CNQ53_RS05335	877050..877535	-	486	WP_000214415.1	antirestriction protein	-
CNQ53_RS05340	877627..878445	-	819	WP_001234381.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T80927 WP_001094445.1 NZ_CP023364:c875736-875359 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T80927 NZ_CP023364:c875736-875359 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT80927 WP_024174331.1 NZ_CP023364:c876194-875826 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT80927 NZ_CP023364:c876194-875826 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Toxin

• AlphaFold DB: A0A454A7U6