Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 73890..74533 Replicon plasmid p80
Accession NZ_CP023361
Organism Escherichia coli strain 1943

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag CNQ54_RS25190 Protein ID WP_001034046.1
Coordinates 73890..74306 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag CNQ54_RS25195 Protein ID WP_001261278.1
Coordinates 74303..74533 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ54_RS25175 69027..69443 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
CNQ54_RS25180 69440..69670 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CNQ54_RS25185 70051..73845 + 3795 WP_001144732.1 hypothetical protein -
CNQ54_RS25190 73890..74306 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
CNQ54_RS25195 74303..74533 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CNQ54_RS25200 74798..75298 + 501 WP_000528931.1 hypothetical protein -
CNQ54_RS25205 75311..76084 + 774 WP_000905949.1 hypothetical protein -
CNQ54_RS25210 76251..77384 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
CNQ54_RS25215 77418..77930 - 513 WP_000151784.1 hypothetical protein -
CNQ54_RS25220 78497..78715 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
CNQ54_RS25225 78717..79022 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD iucA / iucB / iucC / iucD / iutA 1..80602 80602


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T80919 WP_001034046.1 NZ_CP023361:c74306-73890 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T80919 NZ_CP023361:c74306-73890 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT80919 WP_001261278.1 NZ_CP023361:c74533-74303 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT80919 NZ_CP023361:c74533-74303 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References