Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 41544..42103 Replicon plasmid p80
Accession NZ_CP023361
Organism Escherichia coli strain 1943

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag CNQ54_RS24990 Protein ID WP_000421272.1
Coordinates 41828..42103 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag CNQ54_RS24985 Protein ID WP_001387467.1
Coordinates 41544..41828 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ54_RS24955 37007..37447 - 441 Protein_39 zinc-binding dehydrogenase -
CNQ54_RS24965 37848..38738 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
CNQ54_RS24970 38763..39143 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
CNQ54_RS24975 39176..40141 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
CNQ54_RS24980 40187..40939 - 753 WP_000562172.1 IclR family transcriptional regulator -
CNQ54_RS24985 41544..41828 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
CNQ54_RS24990 41828..42103 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CNQ54_RS24995 42209..42502 + 294 WP_001105060.1 hypothetical protein -
CNQ54_RS25000 42537..42665 - 129 Protein_47 IS481 family transposase -
CNQ54_RS25005 42698..43867 - 1170 WP_001072355.1 hypothetical protein -
CNQ54_RS25015 44360..44667 + 308 Protein_49 type II toxin-antitoxin system ParD family antitoxin -
CNQ54_RS25025 45010..45192 + 183 WP_042344623.1 hypothetical protein -
CNQ54_RS25030 45313..46053 + 741 WP_001066953.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD iucA / iucB / iucC / iucD / iutA 1..80602 80602


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T80917 WP_000421272.1 NZ_CP023361:41828-42103 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T80917 NZ_CP023361:41828-42103 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT80917 WP_001387467.1 NZ_CP023361:41544-41828 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT80917 NZ_CP023361:41544-41828 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References