Detailed information of TA system TA080901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1343989..1344614
• Replicon: chromosome
• Accession: NZ_CP023359
• Organism: Escherichia coli strain 1943

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CNQ54_RS06910
• Protein ID: WP_000911330.1
• Coordinates: 1344216..1344614 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CNQ54_RS06905
• Protein ID: WP_000450524.1
• Coordinates: 1343989..1344216 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ54_RS06880	1339792..1340262	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CNQ54_RS06885	1340262..1340834	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CNQ54_RS06890	1340980..1341858	+	879	WP_096034989.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CNQ54_RS06895	1341875..1342909	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CNQ54_RS06900	1343122..1343835	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CNQ54_RS06905	1343989..1344216	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CNQ54_RS06910	1344216..1344614	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CNQ54_RS06915	1344761..1345624	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
CNQ54_RS06920	1345639..1347654	+	2016	WP_000829355.1	tRNA cytosine(34) acetyltransferase TmcA	-
CNQ54_RS06925	1347728..1348426	+	699	WP_000679823.1	esterase	-
CNQ54_RS06930	1348536..1348736	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T80901 WP_000911330.1 NZ_CP023359:1344216-1344614 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T80901 NZ_CP023359:1344216-1344614 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT80901 WP_000450524.1 NZ_CP023359:1343989-1344216 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT80901 NZ_CP023359:1343989-1344216 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33