Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 907527..908181 Replicon chromosome
Accession NZ_CP023359
Organism Escherichia coli strain 1943

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CNQ54_RS04770 Protein ID WP_000244781.1
Coordinates 907774..908181 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CNQ54_RS04765 Protein ID WP_000354046.1
Coordinates 907527..907793 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ54_RS04740 902696..903439 + 744 WP_000951948.1 SDR family oxidoreductase -
CNQ54_RS04745 903496..904929 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
CNQ54_RS04750 904974..905285 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CNQ54_RS04755 905449..906108 + 660 WP_000250274.1 hemolysin III family protein -
CNQ54_RS04760 906304..907284 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
CNQ54_RS04765 907527..907793 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CNQ54_RS04770 907774..908181 + 408 WP_000244781.1 protein YgfX Toxin
CNQ54_RS04775 908221..908742 - 522 WP_001055874.1 flavodoxin FldB -
CNQ54_RS04780 908854..909750 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CNQ54_RS04785 909775..910485 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CNQ54_RS04790 910491..912224 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T80899 WP_000244781.1 NZ_CP023359:907774-908181 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T80899 NZ_CP023359:907774-908181 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT80899 WP_000354046.1 NZ_CP023359:907527-907793 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT80899 NZ_CP023359:907527-907793 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References